-15- 



tobamoviruses when using Azure A (Christie and Edwardson, 

 1977) . 



In fluorescence microscopy, the ORSV inclusions fluoresced 

 when treated with TRITC-con jugated ORSV antiserum (refer to 

 chapter 3). However, they did not fluoresce when either TRITC- 

 con jugated CyMV antiserum or normal serum was used. Expressed 

 sap from ORSV-inf ected plants used in this study reacted 

 homologously in SDS immunodiffusion tests with ORSV antiserum. 

 In the bioassay, ORSV infection caused chlorotic spots on the 

 leaves of Gomphrena globosa as described by Lawson and 

 Brannigan (in press). 

 Cymbidium Mosaic Virus 



Reddish-violet inclusions typical of other potexviruses 

 were found in Azure A-stained epidermal and mesophyll cells of 

 plants infected with CyMV (Christie and Edwardson, 1977). In 

 glutaraldehyde-f ixed tissues, some of these inclusions were 

 banded (Fig. 10). Crescent- or tear-shaped inclusions were 

 also readily found in the Azure A-stained tissues (Fig. 11). 

 Thin sections of banded-body inclusions consisted of large 

 aggregates of tiered (Fig. 12) or whorled, and intertwined 

 virus particles (Fig. 13) like those described previously for 

 CyMV and other potexviruses (Chen et al . , 1983; Christie and 

 Edwardson, 197 7; Hammond and Hull, 1983; Hanchey et al., 1975; 

 Hiruki et al . , 1980; Lawson and Hearon, 1974; Purcifull and 

 Edwardson, 1981 ) . 



CyMV-induced inclusions fluoresced when exposed to 

 TRITC-labelled CyMV antiserum (refer to chapter 3), but not 



