CHAPTER PAGE 



Phase differences 149 



Implication to attractant origin 152 



Conclusions 153 



Lactic Acid Reactions 153 



Altering Attraction 154 



Origin of Attraction 154 



4 APPLICATIONS OF TANDEM MASS SPECTROMETRY 156 



Introduction 156 



Analysis of Multiple Beads Without GC Separation 157 



Daughter Library 157 



Compound Class Screening 158 



Experimental 159 



Daughter Library 160 



Compound Class Screening 162 



Results and Discussion 163 



Analysis of Multiple Beads Without GC Separation 163 



Daughter Library 176 



Compound Class Screening 187 



Conclusions 202 



5 IDENTIFICATION OF SKIN EMANATIONS 205 



Introduction 205 



Sample Introduction and Separation 205 



Cryo-focusing GC 208 



Purge and trap GC 208 



Mass Spectrometry 209 



PCI theory 209 



NCI theory 212 



Characteristic ion fragmentations 217 



Experimental 220 



Identification of Emanations by CI and EI MS 220 



Cryo-focusing GC/MS 220 



Purge and trap GC/MS 223 



Case Study Comparison of Emanations between Subjects .... 224 



Case Study Comparison of Bio-assay to GC/MS Assay 226 



Cryo-focusing GC/MS 226 



Olfactometer 227 



Results and Discussion 228 



Identification of Emanations by CI and EI MS 228 



VI 



