CHAPTER PAGE 



2 SAMPLING METHODS 31 



Introduction 31 



Sampling Considerations 31 



Entomological Sampling 32 



Olfactometer 32 



Field studies 36 



Mass Spectrometric Methods of Sample Introduction 36 



Experimental 39 



Thermal Desorption from a Single Bead 39 



Thermal Desorption from Multiple Beads 40 



Thermal Desorption from Multiple Beads/Cryo-focused GC 



Separation 45 



Purge and Trap/GC Separation 47 



Results and Discussion 49 



Thermal Desorption from a Single Bead 49 



Thermal Desorption from Multiple Beads 64 



Thermal Desorption from Multiple Beads/Cryo-focused GC 



Separation 72 



Purge and Trap/GC Separation 82 



Conclusions 87 



3 STUDIES INVOLVING ATTRACTION 89 



Introduction 89 



Lactic Acid as a Model Compound 89 



Reaction Studies 89 



Altering Attraction 90 



Analysis of Methanolic Perspiration Solution 91 



Experimental 91 



Reactions of Lactic Acid 91 



Altering Attraction 94 



Analysis of Methanolic Perspiration Solution 97 



Results and Discussion 97 



Reactions of Lactic Acid 97 



Characteristic fragmentations 98 



Oligomerization and attachment reactions 108 



Altering Attraction 130 



Addition of acid or base to lactic acid 131 



Addition of acid or base to esters 142 



Analysis of Methanolic Perspiration Solution 142 



