291 

 components are found to increase (or decrease), these should be targeted for testing 

 by bio-assay. 



Another case study related to that described above involves exercise. 

 Attraction of mosquitoes is enhanced (up to a point) during physical exertion and 

 perspiration. Sampling at the proper time interval may yield samples with increased 

 attractant(s) relative to other components in the matrix on the skin. This is 

 speculative; however, should attractants be found in greater concentration on the 

 skin, glass beads could be handled at that time. The bead method would then allow 

 for greater concentration of the attractant(s) while keeping to a minimum the water 

 (from perspiration) adsorbed to the glass, which in turn minimizes water 

 subsequently introduced into the system. 



The case studies examined and discussed thus far have focused on human 

 subjects. Humans are not the sole hosts of mosquitoes. Many species of mosquito 

 feed on multiple hosts. Examining emanations from humans and animals known to 

 attract the same mosquito species may aid in determining the attractants by reducing 

 the components of interest to those present in both sets of emanations. This again 

 is somewhat speculative in that there is no guarantee that specific mosquitoes have 

 only a few types of chemosensilla. There is a possibility that the mosquito may 

 detect different volatile components for each host. 



Tandem mass spectrometry was used only briefly in this work. The studies 

 for chapter 5 yielded many identified components from PPINICI and EI analyses 



