36 

 Animals weights were obtained at the beginning and at the 

 end of the experiment . Blood samples were obtained via 

 jugular venipuncture on d before diet administration and on 

 d 14, 21, 28, 49 and 55. To obtain serum, samples were 

 centrifuged at 700 x g for 25 min, supernatant decanted and 

 frozen until analyzed for Zn and Cu levels. At the end of the 

 experiment on d 55, animals were stunned with a captive bolt 

 shot and euthanized by exsanguination . 



Liver, pancreas, kidney, bone and bone marrow 

 (metacarpus), skin, hoof, leg muscle (flexor carpi ulnaris), 

 and eye were excised and frozen for further mineral analyses. 

 Zinc and Cu in tissues, blood constituents and diet were 



TABLE 4-1. Composition of basal diet 

 offered to sheep (as fed) a 



Ingredient Percentage 



Ground yellow corn 59 



Cotton seed hulls 21 



Soybean meal (44% CP) 12 



Alfalfa meal (14% CP) 3 



Corn oil 3 



Trace mineral salt b 1 



Ground limestone 1 



Vitamins A and D 3 C .0008 



a Zn analysis biweekly indicated means of 415, 444, 

 446 and 421 mg/kg for ZnO, ZnS0 4 , ZnLys and ZnMet, 

 respectively . 



b Provided .24 mg of I (KI), .28 mg of Co (CoC0 3 ), 82 

 mg of Fe (FeS0 4 ), 38 mg of Cu (CuCl), 48 mg of Mn 

 (MnS0 4 ) and no Zn per kg of diet. 

 Provided 5,000 IU of vitamin A and 500 IU of 

 vitamin D 3 per kg of diet . 



