37 

 determined by air acetylene flame atomic absorption 

 spectrophotometry on a Perkin-Elmer Model 5000 with AS-50. 

 Metallothionein was measured in liver, pancreas and kidney by 

 procedures previously described (chapter 3) . 



All data were analyzed using SAS (SAS, 1988) . Repeated 

 measures ANOVA was performed using the general linear model 



(GLM) procedure on changes (increase or decrease from d 1) in 

 serum Zn and Cu concentrations. Tissue Zn and Cu data were 

 analyzed using GLM procedure. In case of significance (P < 



.05) of either serum or tissue mineral concentrations, Waller- 

 Duncan's K-ratio T test was used for multiple comparisons 



(Waller and Duncan, 1969) . 



Results 



Three of the lambs (2 from the control group and 1 from 

 the sulfate group) died from unrelated causes. Weight gains 

 were not different (P > .05) among treatments. Feed intakes 

 were similar for all groups with no anorexia noted. 



When compared to d 1, d 49 serum Zn concentrations had 

 increased by .20 ± .13 (ig/ml (mean ± SEM) for control sheep 

 which was lower (P < .05) than those of ZnLys, ZnS0 4 , and ZnO 

 treatments which had increased by .74 ± .12, .62 ± .07, and 

 .83 ± .12 |ig/ml, respectively, but was not lower than ZnMet 

 which increased by .52 ± .16 fig /ml (means shown on Figure 4- 

 1) . Treatment differences were also seen on d 55 with a 

 higher (P < .05) serum Zn increase for ZnLys (1.58 ± .28 



