55 



Overall, serum CK (Figure 5-4) activity decreased with 

 the beginning of Cu supplementation. Creatine kinase 

 concentrations were highly increased for animals 1 and 77 

 which also showed increased serum Cu and HCT at the time of 

 death. On the other hand animal 4 6 did not show such an 

 increase in CK levels. There also a peak for animal 1 on d 34 

 but it went back to the previous concentration by the next 

 sampling time. 



Overall, GGT concentrations (Figure 5-5) rose in the 

 beginning of the trial but fluctuated throughout for all the 

 wether lambs. Concentrations of GGT rose for the two wethers 

 receiving CuLys on d 27 and remained high until death. The 

 other wether (77) had highly variable GGT level which was low 

 at the time of death. The GGT level for the ewe was 

 relatively constant throughout the experiment . 



Overall, AST concentrations rose from the beginning of 

 the experiment for all the wethers (Figure 5-6) . These 

 concentrations also decreased dramatically before death for 

 two of the animals (1 and 77) and they remained high for the 

 other (46) . 



Heinz bodies determination was positive only for animal 

 77 on d 27 with 41% noted. Another interesting detail was 

 noticed in the blood serum. On d 20 and 23, blood serum for 

 animal 77 had a mustard color to it and on d 27 it turned to 

 red which was indicative of the full blown hemolytic crisis. 

 The animal was found dead by d 30. Furthermore, on d 75, 



