65 



TABLE 6-1. Composition of purified 

 diet fed to rats (As-fed) . a 



Ingredient g/kg 



Egg white solids 200 



Cornstarch 150 



Sucrose 503 



Cellulose 50 



Corn oil 50 



AIN-76 mineral mix b,c 35 



AIN-7 6 vitamin mix c 10 



Biotin t 



Choline bitartrate 2 



a Zn and Cu analysis indicated (mean ± SD) of 



29 ± 2 mg Zn/kg and 5.3 ± .5 mg Cu/kg of all 



supplemented diets . 



b Contains no Zn or Cu . 



e Contents of the mineral and vitamin mix are 



specified in appendix Tables A-l and A-2, 



respectively . 



d Provided .004 g biotin/kg. 



GA) . Seven rats were randomly assigned to each of these 

 treatments . 



Supplemented diets were fed for 4 wks at which point four 

 randomly selected rats from each treatment were sacrificed 

 (first phase). The rest of the animals were fed the basal 

 diet (Table 6-1) for an additional week (second phase) and 

 then sacrificed. Protocol for animal care had been approved 

 by the University of Florida's Institutional Animal Care and 

 Use Committee. All rats were anesthetized by inhaling 



Metafane™ (Methoxyf lurane) and bled by cardiac puncture. 



