66 

 To obtain heparinized plasma, blood was centrifuged at 

 700 x g for 25 min, supernatant decanted and frozen until 

 analyzed for Zn and Cu . Tissues were immediately excised. 

 The liver and both kidneys were frozen at - 80°C, and the rear 

 leg muscles (biceps femoris, vastus lateralis, and gluteous, 

 combined) and bones (femur, tibia, and fibula, combined) were 

 frozen at - 20°C. 



Total MT was measured in kidney and liver by the 109 Cd 2+ - 

 binding method (Eaton and Toal, 1982). The Zn and Cu 

 concentrations in plasma, liver, kidney, muscle and bone were 

 measured by air acetylene flame atomic absorption 

 spectrophotometry on a Perkin-Elmer Model 5000 with AS-50. 



All data was analyzed using SAS (SAS, 1988) . Tissue and 

 plasma Zn, Cu and MT data were analyzed using GLM procedure 

 and in case of significance (P < .05) Waller-Duncan's K-ratio 

 T test was used for multiple comparisons (Waller and Duncan, 

 1969) . 



Results 



Weight gains were not different (P > .05) among 

 treatments or experimental phases, but there was a tendency (P 

 = .07) for CuLys to have a higher ADG than CuS0 4 for phase 1. 

 Diet intakes were similar for all groups for both phases with 

 no anorexia noted. When not mentioned there were no 

 interaction effects (P > .05). 



