METHODS AND MATERIALS 

 Laboratory Trials 



Environmentally Controlled Rearing Conditions 



TM 

 Rearing of immature diptera was accomplished in a Percival 



forced-air, upright growth chamber. The temperature was maintained 



at 29. k C, and continuous lighting was provided by two ^0-watt 



fluorescent bulbs. The growth chamber was modified to include an 



external exhaust system with air supplied to the unit from within the 



laboratory. Cages of adult flies of various species were also kept in 



this growth chamber unless otherwise specified. Whenever a growth 



chamber is mentioned without further clarification in this paper, 



reference is being made to the Percival at 29.^ C, continuous 'lighting, 



and ambient humidity lowered by chamber temperature. 



Some adult flies were kept in a walk-in growth chamber which nad 

 a relative humidity of 35% and a temperature of 26.7 C. Lighting, 

 fluorescent and incandescent, was continuous. Since this chamber was 

 used so infrequently, it will be referred to specifically throughout 

 the text if applicable. 

 Colonization and Rearing of Flies 



Mi'.sca domestiaa (L.) -- the house fly. The laboratory house fly 

 colony was started wi th adults obtained from a poultry farm in Starke, 

 Florida, in October of 1975- Wild flies were placed into 3-3-1 plastic 

 jars half-filled with moistened CSMA ' (Consumer Specialties Manufac- 

 turing Association) and allowed to oviposit. Jars were screened and 



k3 



