kk 



placed in the growth chamber. Pupae were removed from the jars, 

 separated from the CSMA by flotation, and drisd on paper towelling. 

 Care was taken to be sure pupae were free of any mites that may have 

 been attached to the field-collected adult flies. 



Pupae were placed in a standard colony cage (51 X 25 X 25 cm) with 

 four sides and one end covered with window screen. The remaining end, 

 fitted with surgical stockinet, was used as an entryway. Water was 

 provided in a pan ca. 5 cm deep. The water surface was covered with 

 polyfoam chips to provide a resting area for the flies and reduce 

 mortality from drowning. The adult diet, a commercially prepared naso- 

 gastric mixture (Table 5), was thinly spread over a small (ca. 5 X 10 cm) 

 piece of aluminum foil with the edges raised to resemble a shallow pan. 

 Additional diet was added daily in thin layers. This method allowed for 

 a larger feeding surface and reduced waste. Cages with adults were kept 

 in the wa 1 k- i n growth chamber. 



Eggs were collected over 4-hr time periods in moist CSMA from cages 

 where females were an average of 7 days old. CSMA was mixed with water 

 at a ratio of 5:3 and placed loosely in plastic pans 36 cm in diameter 

 and }k cm deep. Eggs, about 1000 per pan, were placed 1 to 2 cm below 

 the CSMA surface to simulate oviposition. Pans were screened and placed 

 in the growth chamber. 



When the colony was well established, a rearing schedule was set up 

 to provide two cages of flies per week for testing purposes. The 

 schedule was based on the average time from egg to pupae at 29.4 C 

 being 10 days. Adults were discarded after 3 weeks. 



Interesting contrasts to the above method of rearing house flies 

 are presented by Grady (1928) and Monroe (1962). 



