132 



and k were left untreated to prevent overlap of treatments. Methoprene 

 (Zoecon 0.k% tech.) sand granules were applied at rates of 538 and 

 1076 mg/m 2 . The weights of granules applied to blocks 3 and 5 (Figure 

 27) were 2.08 and k.]J g respectively. 



Immediately after treatments were applied and 3 days later, samples 

 were collected for laboratory bioassay. Each sample was seeded with 25 

 eggs of M. domestica. 



Resul ts. Mortality produced by methoprene sand granules at 

 1076 mg/m 2 was significantly higher than the mortalities in the other 

 two groups (Table 24). When corrected by Abbott's formula, mortalities 

 produced by the 1076 and 538 mg/m 2 treatments were 9^.3 and kl.3% 

 respectively. 



In the second group of samples, there were significant differences 

 in the mortalities produced by all three treatments (Table 2k). When 

 corrected by Abbott's formula, mortalities produced by the 1076 and 

 538 mg/m 2 treatments were 95.82 and 62.91% respectively. 



Results indicate that methoprene sand granules are effective for 

 at least 3 days post-treatment. When the granules are applied at 

 1076 mg/m 2 , fly mortality of more than 90% can be expected. Field 

 tests, however, may not produce similar results as indicated by the 

 feed-through trials. 



Few pupae were formed in the sample replicates of manure treated 

 with 1076 mg/m 2 of methoprene, because at this high concentration, the 

 larvae were ki 1 led. 



Experiment 2 -- At the University of Florida Poultry Science Farm, 

 methoprene (Zoecon 0.k% tech.) sand granules were sprinkled on manure 

 beneath two treatment blocks of 10 hens caged in pairs. Weights of the 



