134 



granules used for the 1076 and 538 mg/m 2 treatments were 0.57 and 0.29 g 

 respectively. Samples were collected as for experiment 1 above. 



Resul ts. In the first group of samples, house fly mortalities in 

 groups treated with methoprene were significantly higher than the check 

 mortalities (Table 25). When corrected by Abbott's formula, mortalities 

 produced by the 1076 and 538 mg/m 2 treatments were 93-9 and 97.0% 

 respect ivel y. 



In the second group of samples, mortalities in groups treated with 

 methoprene were significantly higher than the check mortalities, despite 

 the high check mortalities. When corrected by Abbott's formula, mor- 

 talities produced by the 1076 and 538 mg/m 2 treatments were 70.6 and 

 88.2°o respectively. 



Results indicate that methoprene sand granules are effective for at 

 least 3 days post-treatment. When the granules are applied at 1076 mg/m 2 , 

 mortalities greater than 90% can be expected, at least on the first day. 

 Results from field tests may differ from laboratory tests as previously 

 stated. It cannot be explained why the lower concentration of methoprene 

 produced numerically higher mortalities in both sample groups. Lack of 

 pupae formation in the 538 mg/m 2 treatment in the second group of samples 

 indicated the presence of a high level of methoprene, possibly due to 

 collection of manure from an area where nonuniform application occurred. 



High check mortalities in both experiments were attributed to the 

 use of house fly eggs in the manure samples. House fly eggs hatch 

 better in wet media and media in this experiment were somewhat dry. 



Laboratory studies with CGA 72662. CGA 72662 (Ciba-Geigy™ 10% 

 soluble concentrate) was added to the diets of six species of flies. 

 The fly species and diets utilized are shown in Table 26. 



