153 



A section of the manure collection area of one house was divided 

 into four blocks and treatments were assigned as shown in Figure 32. 

 A 0.1% solution of CGA 72662 was mixed as shown beiow: 



40 ml of CGA 72662 + 3-84 1 H 2 - 0.U solution 

 The application rate was 3-84 1/9.12 m 2 . Treatment blocks 2 and 3 were 

 tilled twice weekly and blocks and 1 were left until led. 



Pretreatment samples were collected on the day the experiment began 

 and additional samples were collected at weekly intervals. Larvae of 

 house flies, soldier flies, and little house flies were counted as in 

 the previous experiment. Sampling was discontinued after 5 weeks because 

 of climatic conditions. 



Resul ts. Larval means from each treatment block for each sampling 

 period are shown in Table 34. 



The pretreatment house fly samples revealed significant differences 

 between treatment-block population means. Blocks to be treated with 

 CGA 72662 had numerically lower populations than the remaining two blocks. 



One week after treatment, there were no significant differences 

 between the house fly population means in the four blocks, but in the 

 untilled block sprayed with CGA 72662, the house fly population mean 

 was 2.75 as compared to 37-50 in the tilled CGA 72662 block (Table 34). 

 Manure was so wet that house fly larvae were pupating just below the 

 surface of the manure instead of at the edges of the manure pack. In 

 the untilled GCA 72662 block, fly larvae coming up to pupate were being 

 exposed to the concentrated CGA 72662 on the manure surface. In the 

 tilled CGA 72662 block, CGA 72662 may have been diluted by the tilling 

 and was slower acting. 



