838 
To 14 ec. of the liquid (serum + cocain) was added 1} times the 
volume of alcohol 96°/, + 2 drops of HCL. This was centrifuga- 
lized and filtered, the filtrate was turbid. The precipitate was subse- 
quently washed with alcohol and part of the alcohol was evaporated 
down in vacuo. After this the solution was acidified and shaken out 
twice with ether. The ether extract was then acidulated with */,, 
N. HCL to get an aqueous cocain solution. This solution was again 
neutralized with bicarbonas natricus. With this liquid the experiment 
was repeated. 
Exp. 15. We used the liquid of exp. 12 after extracting it with 
alcohol, the amount of cocain was calculated at about '/, °/,. 
Stimulation after: 
2 min. 
4 
So after 40 minutes the 
Reading taken of 
the induct. (E’) 
1.96 
1.96 
1.9 
1.74 
1.68 
1.68 
1.68 
1.1 
(no longer any 
contraction). 
Control (E”) 
1,96 
1.96 
1.96 
1.96 
1,96 
1.96 
1.96 
1.96 
1.96 
1.96 
1.96 
1.96 
1.96 
1.96 
1.96 
1.96 
1.96 
1.96 
1.96 
1.96 
nerve was anaesthetic, from which it 
