839 
appears that through the treatment with acid and alcohol all the 
cocain adsorbed by the serum was detached. (Normal value for 
‘/,°/, cocain is 43 minutes). 
Exp. 16. The liquid used is that of exp. 13 treated with alcohol 
and acid. Here also we found that after 40 minutes the muscle had 
lost its contractility, so that the result coincided with that of expe- 
riment 15. 
In the following experiments we used a stronger solution of cocain, 
Mize o/c /. COCR: 
3 0 
Exp. 17. The liquid is 0.4 ce. 5°/, cocain + 4 ce. Ringer 0.9 °/,, 
consequently */, °/, cocain hydrochloricum. 
18 
08 
a6 
Reading taken of the inductorium 
0.4 
40 50 
60 
minutes 
Fig. 4. Effect of 1/,°/, cocain hydrochloricum solution in Ringer’s fluid (0.6 9/5) on 
the nervus ischiadicus of a muscle nerve preparation of Rana esculenta. 
The result of this experiment is represented by the curve in Fig. 
4. After 28 minutes the nerve was irresponsive, while it appeared 
that, through stimulation with electrode E", the muscle itself had 
remained responsive. Two other experiments yielded the same 
results. 
Exp. 18. Liquid '/,°/, cocain; nerve irresponsive after 30 min. 
Exp. 19. Liquid '/,°/, cocain; nerve irresponsive after 30 minutes. 
Average time in which the nerve becomes irresponsive with '/, °/, 
