843 
(Table continued). 
Stimulating after: | EE Control (E”) 
26 | 1.6 1.9 
28 | 1.52 1.9 
30 1.46 1.9 
32 1.4 1.9 
34 1.4 1.9 
36 1.34 1.9 
38 1.26 1.9 
40 152 1.9 
42 1 1.9 
44 = 
The result of this exp. is similar to that of exp. 26, viz. only 
after 44 minutes irresponsiveness of the nerve. 
The liquid of experiment 27 was treated with alcohol and acid 
as in experiment 15, and was used in experiment 28 (the cocain 
content was calculated at 1°/,). After 22 minutes the nerve was 
no longer responsive from which it appeared that (compare the 
results of experiments 23, 24, 25) through extraction with alcohol 
the cocain had been detached. 
We now considered the question whether the behaviour of brain- 
substance toward cocain is similar to that of serum. To 5 grms of 
rabbit’s brains was added 10 ce. of a 2°/, solution of cocain in 
Ringer 0.6°),. After standing for 30 minutes at room-temperature it 
was centrifugalized and the supernatant fluid was examined. A 
control experiment was made on 5 grammes of brains and 10 cc. 
of Ringer without cocain. The latter liquid proved to be indifferent 
to the nerve. 
Erp. 29. Liquid: 5 grms of rabbit's brain-substance and 10 ec. 
2°/, cocain; contains 1,33 °/, cocain (see Table Exp. 27). 
So it appears that after 50 min. the nerve has become anaesthetic. 
Since in the normal experiments with 1°/, cocain anaesthesia appears 
after 20 minutes, we must conclude that also brain-substance inhi- 
bits cocain. 
Exp. 30. Repetition of experiment 29 but with cat’s brains, 
