846 
Exp. 31. Liquid: brain-substance + cocain after treatment with 
hydrochloric acid and alcohol, computed at 1 °/, cocain hydro- 
chloricum. 
Stimulation after: Reading taken of Control 
2 1.8 1.8 
4 1.8 1.8 
6 1.7 1.8 
8 1.68 1.8 
10 1.6 1.8 
12 1.5 1.8 
14 1.44 1.8 
16 1.4 1.8 
18 1.4 1.8 
20 1.3 1.8 
22 1.1 1.8 
24 — 1.8 
Here, then, the cocain action manifests itself again, for the nerve 
is irresponsive after 24 minutes, so that no cocain has been decom- 
posed by the brain-substance. 
In order to show that from brain-substance, after extraction with 
hydrochloric acid and aleohol, no materials are abstracted which, 
of themselves, are deleterious to the nerve, so that thereby in ex- 
periment 31 the cocain action might have been intensified, we under- 
took a control exp. 32, in which a liquid was added to the nerve 
that was composed of 5 grms. of cat’s brains and 10 c.c. RINGER 
0.6 °/, and then extracted with hydrochloric acid and alcohol. 
This liquid again proved to be indifferent to the nerve, because 
within an hour the responsiveness had not diminished. 
Exp. 33. This experiment is a repetition of exp. 31. 
Liquid: Cat's brain-substance and cocain-solution equal to 1 °/, 
cocain hydrochloricum. 
After 54 minutes the nerve is irresponsive, which again shows 
that the cocain action is inhibited by brain-substance. 
Exp. 34. The liquid of exp. 33 was again treated with alcohol 
and hydrochlorie acid. 
