965 
cavity; besides the undeniable resorption, there is also a strong emigration. 
(Moreover it is also mentioned by WeEIDENREICH !) that the injection with salt 
solution can cause emigration). How strong this emigration actually is, will appear 
from the fact that it is often possible after centrifugation of the fluid tapped off, 
to obtain 1—3 cc. of pure leucocytes as a reaction upon the double injection, ie. 
more white blood corpuscles than there are at any moment present together in 
the blood of the rabbit. 
The cells of the exudation apart from variations of minor character, were 
constantly the same. The mononuclear transsudate cells were still in the majorily 
but sparcely present if the fluid was drained off about 3 hours after the first 
injection. There were then hardly any granulated cells present; on the other hand 
the cell-type was totally reversed if the fluid was tapped off in the usual way 
after the second injection. The quantity of cells was then increased many fold 
and consisted almost exclusively (+ 95°/,) of polynuclear granulated cells of the 
pseudo-eosinophile type such as are present in the blood of the rabbit. 
On the ground of several indications it was concluded that the mononuclear 
which occur first, owed their origin to a local reaction by disquamation of 
epithelial cells, and, that only the granulated cells emigrated from the blood. 
Further it appeared that the duration of life of these granulated cells as such 
outside the blood could be only exceedingly short, and that in this case there is 
practically no question of any functions of living cells. This appeared f.i. from the 
fact that upon the introduction of starch grains or fat into the abdominal cavity 
where an exudation was already present, all cells were precipitated with the starch 
and without acting upon it were almost quantitatively destroyed and surrounded 
with formative tissne after a few days. If some days after the injection, the abdo- 
minal cavity is again rinsed, then these are again found principally mononuclear 
cells óf which very many have taken up into their protoplasm one or more 
granulated cells. Thus to some extent a state of rest has set in again. The rabbit 
seems normal again; only on the first day it was a little out of sorts. This animal 
and also other kinds of animals used (goat guinea pig) responds as regards its 
health only very slightly; Even a prick in the bowels is endured without further 
effects. 
Still the condition of the rabbit once used is not what is has been originally. 
This is apparent when after a week or a few weeks we inject it anew; already 
a few hours after the first injection we notice an exudation consisting nearly 
exclusively of polynuclear cells. 
What was found during the progress of the exudation, was for the greater 
part in agreement with what other researchers held in this connection. It is known 
that the neutrophile granulated leucocytes when out of the blood tend to degenerate 
and the mononuclear to regeneration. But still as a rule the idea is entertained 
that, out of the blood the polynuclear cell carries out important functions as a 
living organism; This can be denied on the grounds of our investigations. We do 
not aim at belittling the very important functions of these cells as carriers of 
organic matter such as, for instance, ferments. 
It was in continuation of these investigations, that we traced how 
the circulating blood of the rabbit responded to this emigration by 
milliards of the polynuclear cells. This appeared to be of such a 
') Die Leukozyten und verwandte Zellformen. Wiesbaden 1911, p. 385. 
