Water for Bacteriological Analysis. 23 
and closing glass stoppered bottles under water. My 
apparatus, though a modification of Prof. Ellis’, contains 
improvements of my own which render it specially adapted 
to taking large numbers of samples by making it simpler 
in construction and more rapid and accurate in action. 
All who have worked at water analysis know the great 
importance of making a very large number of separate 
observations before drawing conclusions. 
My outfit consists of one collecting frame, shown (reduced 
to one-half its linear dimensions) in Plate III., into which 
the bottles can be successively fitted. It was made under 
my direction by Mr. O. Wendell, of 170 Coursol Street, 
Montreal, and cost about eight dollars. It may be briefly 
described as a sinking frame, to which the bottle is attached 
by a fixed clamp, while a movable clamp is used to raise 
and lower the stopper. 
The frame is made of brass and has for its base a hollow 
cylindrical box D, 24 inches deep and 2 inches in diameter. 
The box contains two pounds of shot and can be filled at a 
small hole EK, which is closed by a screw. Attached to the 
top of this box are two flat brass bars F F, in the upper part 
of which a slot is cut allowing the movable cross bar A 
sufficient vertical play (1 inch) to admit of the bottle being 
opened beneath the water. 
The neck of the bottle is grasped at B by a brass clamp, 
the jaws of which are lined with soft rubber, fastened on by 
rivets. These jaws work on pivots and are attached to the 
upright bars F. F. by means of a brass rod bent outward so 
as to bring the neck of the bottle into the line of traction. 
The pivots allow some lateral play. The clamp is kept 
closed upon the neck of the bottle by a brass spring C made 
of No. 18 wire. 
The stopper H of the bottle is in the form of a tapering 
glass rod which is grasped by another clamp K and kept 
closed by the brass spring C. This clamp is secured to the 
sliding cross bar A by a horizontal pin working in slots 
which allow of sufficient backward and forward play to per- 
mit the stopper to adjust itself to the bottle. At the point 
