130 



refractive index at iS° C. = 1-4744. The freshly-distilled oil was soluble in one 

 vohime 90 per cent, alcohol. The saponification number was 35-7, equal to 12-49 

 per cents, of ester as born\l- and geranyl-acetates. 



The oil obtained from the general material was taken for the full inx-estiga- 

 tion. It had specific gra\-ity at 18° C. = 0-8729 ; rotation ((,, = 4-27-9°; refrac- 

 tive inde.x at iS° C. = 1-4747. The freshly distilled oil was scarcely soluble in 

 ten volumes of 80 per cent, alcohol, but was not rendered turbid by excess ; it 

 was readily soluble in one \-olume 90 per cent, alcohol, but rapidly became less 

 soluble on keeping. The saponification number was 47-03, equal to i(v46 per cent. 

 of ester. In the cold with alcoholic potash, and with three hours contact, the 

 saponification number was 24-5, equal to 8-57 per cent, of ester. 



On redistilling, practically nothing came oxer below 156° C. ; between 

 156° and 160,° 30 per cent, distilled; between 160° and 175° C, 45 per cent.; 

 between 175° and 200° C, 8 per cent. ; between 200° and 230° C, 12 per cent. 

 The specific gravity of the first fraction at fg" C. = 0-8562 ; of the second, 0-8571 ; 

 of the third, o-868g; of the fourth, 0-9415. The rotation of the first fraction 

 a„ = -|- 30-4 ; of the second, + 27-2° ; of the third, -f 2i-o° ; of the fourth, + 32-4. 

 The fourth fraction contained 68-2 per cent, of ester. Both borneol and acetic 

 acid were isolated and determined ; so that the high activity is largely due to the 

 presence of dextro-rotatory bornyl-acetate, and to dextro-rotatory borneol also. 

 The refractive index at 21° C. of the first fraction = 1-4733 ; of the second, 1-4736; 

 of the third, 1-4744; of the fourth, 1-4723. 



Terpenes. — The first and second fractions were mixed together and redis- 

 tilled. Between 156° and 160° C. 42 per cent, distilled, arid 29 per cent, between 

 160° and 161° C. The specific gravity of both fractions at 20° C. = 0-8549; the 

 rotation of first fraction «„ — 4-30-8°, or a specific rotation [«],, -f- 36-02° and 

 the refractive index at 20° C. = i'4733. The nitrosochloride was easily prepared 

 from this fraction, and was finally purified from chloroform by precipitating with 

 methyl alcohol. The nitrosopinene was prepared from this, and when finally 

 purified from acetic ether it formed good crystals which melted at 132° C. The 

 low boiling terpene in the leaf oil of this species is, therefore, dextro-rotatory pinene. 

 The second fraction also consisted largely of this pinene. The third fraction 

 ('i75''-200° C; consisted largely of dextro-rotatory limonene together with di])entene. 

 The presence of these terpenes in the leaf oil of this species was completely proved 

 in the oil obtained from the material from P>o])py Mniiiitain, No. 2. 



Alcohols. — That portion of the oil distilling between 200°-230° C. was taken 

 for the determination of the alcohols and tlie acids of the esters, i-ogi gram 

 of oil reg. 0-2128 gram potash, S.N. = 195-05, equal to 68-26 percent, ester. The 

 remainder was saponified by boiling in aqueous potash, and the oil\ iKuliini 

 separated. This oil had a marked odour of borneol. SnHieienf Ixniieol was 



