425 



pinene, and 60 per cent, between 155-159°. These fractions gave the following 

 results : — 



Eoiling Point. 



CO. d^-C. 



i 



"'D I dcm. tube. 



Refractive Index 

 at 24° C. 



155-156' c. 

 155-156" c. 



156-159° C. 



50 

 32 

 18 



0.8546 



0-8545 

 0-8546 



- 20-5° 



- IQ-6° 



- i8-2^ 



1-4727 

 1-4733 

 1-4735 



The fractions had the appearance and odour of pinene, the first particularly 

 so. The nitrosochloride was prepared with the first two fractions, and when this 

 was finally purified by dissolving in chloroform and precipitating with methyl 

 alcohol, it melted at 108° C. The nitrosopinene was prepared from this in the 

 usual way, and when finally purified from acetic ether it melted at 132° C. 



The principal constituent in this oil is thus pinene, the results pointing 

 to the absence of the other members of the terpene group. 



The (?) Sesquiterpene. 



The fourth fraction of the first distillation was again distilled, and 5 c.c. 



obtained boiling between 285-295° C. This oil was lemon-yellow in colour, and is 



evidently the constituent which gives the colour to the crude oil. Its specific 



gravity at 24° C. = 0-9209; rotation a„ = -|-3-4°; and refractive index at 23° C. 



= 1-5065. 



When dissolved in chloroform and shaken with a few drops of sulphuric 

 acid, the colour soon became of a deep red, darkening quickly to red-brown and 

 crimson ; it still had a crimson tint after twenty-four hours, but after two days 

 the colour inclined to violet, which colour remained constant for some days. 



When dissolved in chloroform and one drop of bromine added, the solution 

 was instantly discoloured; on adding more bromine the colour quickly changed 

 to green, soon becoming deeper in colour and then blue-green ; in half an hour 

 the colour was indigo blue, this blue colour remaining constant for days. This 

 substance is thus active to light, is unsaturated, boils at a high temperature, and 

 has the refractive index and specific gravity corresponding closely to the require- 

 ments for a sesquiterpene. When obtained in larger quantity it will be again 

 investigated. 



The Diterpene. 

 The residue left in the flask on the first distillation was poured into a basin 

 and allowed to crystallise ; after two days a semi-crystalline mass had formed, 

 and this was spread on porous plates and allowed to remain for five days, by which 



