4 BULLETIN 517, U. S. DEPARTMENT OF AGRICULTURE. 



In this second test, while the concentrated filtrate gave satisfactory 

 results, the reaction was not as marked as in the case of the first 

 fowl, on which the nonconcentrated fluid was used. 



On June 22 a third series of trials with the filtrate was conducted 

 upon 6 fowls that had been inoculated intravenously on May 27 

 with a culture of Bacteriunfi pullorum. 



Fowls 77, 78, and 81, with two controls, 86 and 87, received in the 

 right wattle 0.1 c. c. of the same concentrated filtrate as used before. 

 In each case the injected wattles showed some edema within an hour. 



On June 24 fowls 77 and 87 still showed slight edema, while fowl 

 81 showed a considerable swelling. The results of these tests are 

 shown in Table II, together with data on agglutination test, autopsy, 

 and cultures from ovaries made at a later date. 



Table II. — Concentmted filtrate tested on foirls 26 days after inoeulation. 



Fowl No.— 



Edema 



after 1 



hour, 



June 22. 



Edema 

 after 24 

 hours, 

 June 23. 



Edema 

 after 48 

 hours, 

 June 24. 



Autopsy. 



CultiKe. 



Agglu- 

 tination, 

 1 : 100. 



77 



Slight... 



Slight... 

 Slight... 

 Slight... 



Slight... 



-t- 

 Slight... 



+ 



-t- 



■? 







+ 



-t- 



+ 







+ 

 + 



-1- 







78 



81 



Sr, 1 



871 



...do.... 

 ...do.... 

 ...do.... 

 ...do.... 



1 Controls. 



At the same time that the foregoing test was made a similar one 

 Avas conducted with the original nonconcentrated filtrate. 



Infected fowls 76, 79, and 82, together with fowls 88 and 89, sup- 

 posedly noninfected controls, were injected in the right wattle with 

 0.1 c. c. of nonconcentrated filtrate. All showed a discernible edema 

 shortly after injection. 



On June 23 a slight edema persisted in fowls 76, 79, 82, and 88 

 (control). On June 24 fowl 88, the control, still showed a slight 

 edema, fowl 76 showed a slight reaction, while fowls 79 and 82 

 showed swelling indicative of a fair positive reaction. The results 

 of these tests and other data are given in Table III. 



Taule III. — Nonconcentrated filtrate tested on foicls 26 days after inoculation. 



Fowl So. — 



Edema 



after 1 



hour, 



June 22. 



Edema 

 after 24 

 hours, 

 June 23. 



Edema 

 after 48 

 hours, 

 June 24. 



Autopsy. 



Culture. 



Aggluti- 

 nation, 

 1:100. 



7(1 



Slight. 

 Slight. 

 Slight. 

 Slight. 

 Slight. 



S'ight. i Slight. 

 Slight. 1 + 

 Siight. -1- 

 Slight. Slight. 



-1- 

 + 

 ? 



+ 



X 



+ 



4- 



-1- 



79 



-1- 



82 



+ 



88' 



-1- 



8a ' 













1 Controls. 



