TEST FOR BACTERIUM PULLOBUM INFECTION IN FOWLS. 5 



Comparing the results shown in Tables II and III, it would ap- 

 pear that the nonconcentrated filtrate has up to date given the most 

 satisfactory results. 



On August 16, 1915, tests of the original nonconcentrated filtrate 

 were made. Fowls 73, 76, 77, 78, 79, 81, and 82 were injected with 

 0.1 c. c. in the left wattle. Of these, fowls 73, 81, and 82 showed re- 

 actions at 48 hours. These had reacted previously, fowl 73 on June 

 9, and the other two on June 22. 



Fowls 76, 77, and 78 did not react from the injection on August 16, 

 and it is noted that they had not reacted well from the injection on 

 June 22. At that time fowl 78 was negative and fowls 76 and 77 

 were rated as slight. Of four controls, fowls 95, 96, 97, and 98, 

 injected with filtrate on August 16, one, fowl 95, gave a reaction. 



Table IV shows the present test, together with previous and subse- 

 quent tests on these birds. For convenience the table includes the 

 results of two succeeding tests on some of these birds, together with 

 the results of the agglutination test, autopsy, and cultures from 

 ovaries made at a later date. 



Table IV. — Retests of foivls icith various test products. 



Fowl 



No.— 



p 











1 



03 



§ 



s 

 1 



aS 





1 



< 



a 

 



d 

 



8 

 a 





June. 



June. 



June. 



Aug. 



Sept. 



a 





10 



11 



23 



24 



23 



24 



17 



18 



3 



4 



21 



22 



"So 



< 



73. 



+ 



+ 











+ 



+ 

 + 

 + 



+ 



-1- 

 + 

 + 



+ 



+ 



+ 



+ 



+ 

 + 

 + 

 + 

 + 

 ? 

 ? 

 + 



+ 

 + 

 -1- 

 + 

 + 



+ 



+ 



76 



Slight. 



Slight. 







-|- 



77. 1.. . 





Slight. 



Slight. 



Slight. 



Slight. 



SUght. 

 -1- 



+ 

 + 

 + 



Slight. 



Slight. 



+ 



Slight. 



+ 



78 1 









+ 



79. . 





Slight. 



+ 







4- 



81 : 





Slight. 



-1- 







+ 



82 







Slight. 



+ 







+ 



951. ~ 















-|- 



961... 

























971... 

























981... 



. . 



















....|.... 







1 



;■' ! 







1 Controls. 



The fact as shown in the tables that three controls, fowls 87, 88, 

 and 95, gave slight reactions, suggested the idea that an edema per- 

 sisting for 24 hours or longer after injection might possibly be 

 induced by causes such as irritation from the carbolic acid in the 

 test fluid or puncture by the hypodermic needle. In order to deter- 

 mine this point, 6 fowls were tested, as follows : Tw^o were injected 

 in the wattle with 0.1 c. c. of plain bouillon, 2 with 0.1 c. c. of 

 bouillon carbolized to the same degree as the culture filtrate, and 2 

 received the hypodermic-needle puncture only. No reaction occurred 



