TEST FOR BACTEEIUM PULLOKUM INFECTION IN FOWLS. 7 



On September 2, fowls 73 and 77 and control fowl 186 were in- 

 jected with 0.1 c. c. As shown in Table V edema was present at 

 21 hours in fowls 73 and 77 and entirely absent in control fowl 186. 



On September 20 the following birds were injected in the wattle 

 with 0.1 c. c. of the same bacterial product as used in the preceding 

 test: Fowls 73, 77, 78, 79, 81, 82, and controls 55, 74, 88, 89, 90, 92, 

 97, 98, and 99. The results of the test are shown in Table VI. 



Table YI. — Further tests with killed bouillon cultures. 



Fowl No.— 



Edema 

 after 24 

 hours, 

 Sept. 21. 



Edema 

 after 48 

 hours, 

 Sept. 22. 



Autopsy. 



Culture. 



Aggluti- 

 nation, 

 1 : 100. 



73 



+ 

 Slight... 



+ 

 + 

 + 

 + 



+ 



+ 



+ 

 Slight. . . 



do 



+ 



+ 

 Slight... 



Slight. . . 



+ 

 + 

 + 

 + 

 ? 

 ? 



6 



+ 





 



+ 

 + 

 + 



+ 







+ 

 

 



+ 



77. 



_i- 



78 



+ 



79 



+ 



81. . 



+ 



82 ■. 



+ 



551 



Not 



74 1 



tested. 



881 . 



+ 



891 





90 1 



Not 



921 



tested. 

 Do. 



991... 









1 Controls. 



On September 23, the following fowls were killed by bleeding 

 and blood was saved for agglutination tests. Autopsy notes follow : 



Fowl 73. A hard tumorlike mass about an inch in diameter is attaclied to 

 the ovary by fibrous threads and vessels. Other small ova show typical ap- 

 pearance of pullorum infection. Baeterium pullorum was recovered in pure 

 culture from the ovary. 



Fowl 77. Ovary contains one ovum the size of a pea, having the appearance 

 of an old pullorum ovum. B. pullorum was obtained in pure culture. 



Fowl 78. One ovum the size of a pea and having characteristic color of those 

 with pullorum infection. Also several smaller similar ones and a cyst the size 

 of a hickory nut which is filled with a colorless fluid. B. pullorum was obtained 

 in pure culture from the ovum. 



Fowl 79. Ovary contains a number of typical pullorum ova. Pure culture 

 of B. pullorum was obtained. 



Fowl 81. Ovary contains large ova apparently normal. There are some 

 small brownish ova that may or may not be infected. Liver contains a few 

 necrotic spots. Cultures from both liver and ovum gave negtive results. 



Fowl 82. Large ova apparently normal. Some small brownish ova that may 

 or may not be infected. Liver contains a few whitish spots. Cultures from 

 both liver and ova gave negative results. 



On September 24, the following control birds were killed by 

 bleeding, and serum was saved for agglutination tests: Fowls 86, 

 74, 88, 89, 97, 98, and 99. All Avere normal, and cultures were nega- 

 tive except for fowl 88, which had reacted to the wattle test. This 



