8 BULLETIN 517, U. S. DEPARTMENT OF AGEICULTUEE. 



bird was found to possess a typical piilloriim ovary, and yielded a 

 pure culture of Bacterium indlorum. 



In preparation for another set of trials of the test upon artificially 

 infected fowls, on August 26, 1915, the following birds received 

 intravenous injections of 1 c. c. of bouillon culture of nine strains 

 of B. jmUorum mixed: Fowls 28, 67, 85, 182, 294, 204, and 215. 

 On September 7 the folloAving birds were injected intraabdomi- 

 nally with 5 c. c. of a similar mixture of strains: Fowls 16, 18, 

 32, 34, 36, 37, 38, 39, 45, 46, 47, 48, 49, 64, 65, 69, 76, 178, 190, and 

 197. Fowls 28, 85, and 215 died shortly after the injection. 



The birds were tested with killed bacterial culture. The results 

 of the two tests, with autopsy findings and results of cultures inocu- 

 lated from the ovaries, are given in Table VII. 



Table VII. — Ttoo tests with nonconcent rated l-iJled evltnre. 



Fowl No.— 



49.- 

 64-. 

 65.. 

 67.- 

 69.. 

 176- 

 178. 



197.. 



182.. 

 204.. 

 291.. 

 41 '.. 

 421.. 

 771.. 

 278'. 

 179'. 

 80'.. 

 181 '. 

 101 ' . 

 102'. 

 103 1 . 



Dec. 



7-8. 



Feb. 



9-10. 



4- 



+ 



+ 



_ 



+ 



_ 



— 



— 



+ 



+ 



+ 



+ 







Trace. 



— 



+ 



+ 



Trace. 



— 







+ 



— 



+ 



+ 



+ 



Trace. 



+ 



+ 



+ 



— 



+ 



+ 



+ 



— 



+ 



+ 



Trace. 



— 



+ 



4- 



+ 



+ 



+ 



+ 



— 



— 



+ 



+ 



Trace. 



— 



+ 



+ 



+ 



— 



+ 



— 



— 



— 



+ 



+ 



Trace. 



— 



+ 



_ 



+ 



— 



+ 



+ 



+ 



— 



+ 





+ 



Trace. 



+ 



-h 



+ 



Trace. 



+ 



— 



— 



— 



+ 



■ + 



+ 



Trace. 



+ 



+ 



+ 



Trace. 



+ 





Trace. 



I 



+ 



- 



- 



- 



- 





- 



- 



Autopsj- Mar. 16. 



Questionable 



Positive 



do 



do 



Questionable 



do 



Positive 



Normal 



Cult- 

 ures 



4- 

 + 



do 





do 





Positive 



Normal 



- 



Questionable 



Positive 





Normal 





Positive 





Questionable 



Positive 



+ 

 + 



do 



do. 





do. . . 





do.. 





Questionable 



Normal 



- 



Not killed 





do 





Normal 





Died .... 





Normal . ... 





Not killed 





do 





do 





do 





..:..do 









' Controls. 

 SUMMARY OF THE TESTS WITH ARTIFICIALLY INFECTED BIRDS. 



In the course of the experiments recorded in the foregoing, 32 

 birds that had been exposed to infection by injection^ of live cul- 

 tures were employed. "When tested for the first time 29 of these, or 

 90 per cent, revealed edematous swellings rated as either slight or 

 positive at 24 hours after injection with the diagnostic agent. When 

 read at a 48-hour interval, 23, or 71 per cent, of the same birds gave 



