TEST FOR BACTEEIUM PULLOEUM INFECTION IN FOWLS. 



11 



of every degree of swelling, without implying that the slighter 

 swellings were significant. 



Table YII. — Comparison of intradermal and agglutination tests. 



Fowl 

 No.— 



93 

 76 

 77 

 73 

 72 

 96 

 29 

 59 

 81 

 100 

 97 



Intradermal test. 



Whole wattle swollen, X3; droops 



Whole wattle swollen, X3 



Lower half swollen, X3 



Swollen, X2 - . . 



Swollen, X2 



Swollen, X2 



Lower half swollen, X2 



Lower half swollen, Xl-5 



Lower half swollen, xl-5 



Lower half swollen, Xl-5 



Lower half swollen, Xl-5 



Aeglu- 





tina- 



Fowl 



tion 



No.— 



test- 





_ 



66 



+ 





+ 



52 



(?) 



99 



- 



60 



- 



95 



- 





Intradermal test. 



Swelling and drooping of feathered 

 sldn at edge of wattle , 



Trace of swelling at lower edge of 

 wattle 



Swelling possibly due to trauma- 

 tism, as wattle is very blue 



Questionable swelling of feathered 

 skin at edge of wattle 



Trace of swelling on posterior half 

 of wattle 



Agglu- 

 tina- 

 tion 

 test. 



?+ 



The results w^ere particularly discordant in the case of fowl 93, 

 which had been placed at the top of the list as showing the best 

 intradermal reaction, while it failed to give a reaction to the agglu- 

 tination test. In view of the discrepancy. Dr. Eettger obtained the 

 bird in question, together with three others, for retest and autopsy. 

 The results are shown in Table IX : 



Table IX. — Comparison of retests and autopsy findings. 



Fowl 

 No.- 



Intradermal test . 



Agglutina- 

 tion test. 



Condition of 

 ovaries at 

 autopsy. 



24 hours. 



48 hours. 



93 



72 



29 



60 



Slight 



Swollen Xl.5..-. 



— 



Normal, 

 Do. 

 Normal (small). 

 Normal. 



Swollen, X2 



_ 



The result of the retest and autopsy of birds 93 and 72 is not 

 wholly satisfactory. The repetition of the reaction in both cases is 

 significant ; but, on the other hand, the results of the agglutination 

 test and autopsy leaves the matter inconclusive. As to the remain- 

 ing discrepancies in Table VIII, the many other cases noted as surely 

 the result of freezing indicate that it is not desirable to apply the 

 intradermal test where there is a possibility of freezing. 



trials by other individuals. 



In several instances the test product was sent to interested indi- 

 viduals on request. One report on the results was received in which 

 1,301 birds were tested and T8 gave a positive reaction. The latter 

 were retested by the agglutination method, and TO gave a positive 

 reaction. 



