4 



BULLETIN 915, U. S. DEPARTMENT OF AGRICULTURE. 



RESULTS OF EXPERIMENTAL WORK. 



The results from the subcutaneous and oral administration of 

 barium chlorid are summarized in Tables 4 and 5, respectively. 



Table 4. — Toxicity of barium chlorid to brown rats (injected subcutaneously on the 



abdomen) . 



Num- 

 ber of 

 rats. 



Barium 

 chlorid 

 in 0.65 

 per 

 cent 

 NaCl 

 solu- 

 tion. 



Ba- 

 rium. 



Ba- 

 rium 

 chlorid. 



Fatalities. 



10th 

 hour. 



24th 

 hour. 



Later. 



Num- 

 ber. 



Total 

 per 

 cent. 



2 

 7 

 3 

 5 

 1 

 9 



Per cent. 

 0.45 

 .45 

 .45 

 .45 

 .45 

 .45 



Mg.per 

 kilo. 

 20 

 25 

 35 

 50 

 75 

 100 



Mg.per 

 kilo. 

 35 

 45 

 62 

 89 

 133 

 178 





 

 1 

 1 

 1 

 8 





 

 1 

 

 

 





 3 

 

 3 

 

 







3 



4 



1 

 8 





 43 

 67 

 80 

 100 

 89 



Table 5. — Toxicity of barium chlorid to brown rats (injected by stomach tube after pre- 

 liminary light etherization). 



Num- 

 ber of 



rats. 



Ba- 

 rium 

 chlorid. 



Ba- 

 rium. 



Ba- 

 rium. 



Fatalities. 



16th 

 hour. 



24th 

 hour. 



Later. 



Num- 

 ber. 



Total 

 per 

 cent. 



Aver- 

 age per 

 cent. 



6 

 2 



2 



5 

 2 

 5 



Per 

 cent. 

 3.5 

 1.7 

 3.5 

 1.7 



L7 



3.5 



Mg. per 

 kilo. 

 100 

 200 

 200 

 250 

 250 

 300 

 300 



Mg. per 

 kilo. 

 178 

 355 

 355 

 445 

 445 

 533 

 533 



1 

 



4 



2 

 5 





 

 

 

 

 

 



ooooooo 



1 





 4 

 1 

 4 

 2 

 5 



17 

 

 80 

 50 

 80 

 100 

 100 



17 



} « 



} » 



\ 100 



The subcutaneous lethal dose was from 45 to 89 mg. per kilo, and 

 the oral lethal dose from 355 to 533 mg. per kilo. From this it 

 would appear that barium chlorid is about six or seven times more 

 toxic subcutaneously than per os. 



The data obtained from the administration of barium carbonate 

 in starch paste suspension by a stomach tube to etherized rats are 

 given in Table 6. 



Table 6. — Toxicity of barium carbonate, 100 fine, suspended in starch paste (injected by 

 tube after preliminary light etherization). 





Num- 

 ber 

 of 

 rats. 



Barium 

 carbon- 

 ate in 

 suspen- 

 sion. 



Barium. 



Barium car- 

 bonate. 



Fatalities. 



Kind of rats. 



16th 

 hour. 



24th 

 hour. 



Later. 



Num- 

 ber. 



Total 

 per 

 cent. 



Aver- 

 age 

 per 



cent. 





16 

 7 

 6 

 7 

 7 

 7 

 7 

 16 

 17 



P. ct. 

 10 

 10 

 10 

 20 

 20 

 •20 

 10 

 10 

 10 



Mg. per kilo. 

 350 

 400 

 400 

 525 

 525 

 525 

 700 



830 to 1,400 

 1,400 



Mg. per kilo. 

 500 

 630 

 630 

 750 

 750 

 750 



1,000 

 1,200 to 2, 000 



2,000 



3 

 5 

 2 

 4 

 2 

 3 

 5 

 14 

 15 





 

 

 1 

 2 

 1 

 2 

 

 





 1 

 3 

 1 

 2 

 3 

 

 1 

 



3 

 6 

 5 

 6 

 6 

 7 

 7 

 15 

 15 



19 

 86 

 84 

 86 

 86 

 100 

 100 

 94 

 88 



19 



Do 



White 



| 85 





1 



Black.. 



\ 90 



White 



1 





1 



Do 



\ 92 



Do 



1 







