RESEARCH METHODS IIST STUDY OF FOREST ENVIRONMENT. 165 



piration of the leaf. In order to refer this to a definite standard, it is 

 compared with the time required by the strip to make the same change 

 over a free-water surface blanketed by one millimeter of air at a tem- 

 perature the same as the leaf. The reaction time of each slip must 

 therefore be ascertained under standard conditions. The apparatus 

 for making these standardizations is described in detail by Livings- 

 ton and Shreve (161). 



It would obviously be a great labor to determine the reaction time 

 of each slip for every temperature likely to be used ; but this is un- 

 necessary, because if the time is determined for one temperature it 

 can be easily derived for any other, since it is inversely proportional 

 to the pressures of saturated aqueous vapor at the different tem- 

 peratures. 



The chief objection to the method is the large personal equation in- 

 volved. The fading of the slip is very slow indeed, and no perceptible 

 change may occur for many seconds. Practically the system seems to 

 work out well and yields much more consistent results than potom- 

 eters. It is easily used in the field and in all kinds of sites, and ap- 

 pears to be a valuable addition to field research methods. 



Method of excised twigs. 



In measuring transpiration by means of cut twigs placed in 

 potometers. the actual amount of water given off per unit of leaf 

 surface can be determined. 



The apparatus used consists of a flask closed with a two-hole, rub- 

 ber stopper, in one hole of which is inserted a glass tube bent at right 

 angles: in the other the twig is sealed. The horizontal section of the 

 gla-s tube is graduated so that it may be used to measure the Avater 

 removed by the twig, and is drawn to a fine point at the end to mini- 

 mize evaporation from the end of the tube. The twigs must be cut 

 off under water and the ends must be kept continuously wet, or large 

 variation- in transpiration will occur. The sealing into the potom- 

 eters must be very carefully done also, as the least leak will totally 

 vitiate results. This method is particularly useful when it is de- 

 sirable to determine transpiration of several species or in several sites 

 simultaneously. The results tend to be erratic, however, and ac- 

 cordingly the determinations should !»<• made in duplicate, a! least to 

 avoid gross errors. The same twigs can only be used for a short time, 

 a fter v. nidi fre-li ones must he obtained. 



To det< rmine the leaf area, as must !><■ done to reduce (lie lnins|)i- 

 ration \<> amount per unit surface, the simplest method is to cut a 

 number of piece9 of known area with ;i cork cutter, or preferably a 

 centimeter punch, weigh them, and then weigh the residue of the 

 leaves and figure their area proportionately. 



