SACBROOD. 



15 



verse tracheae under slight magnification may be distinctly seen. 

 The narrow, somewhat transparent area present along the ventral 

 median line of the healthy larva is stOl to be seen in this stage of the 

 decay. The lateral and posterior margins are still deeply notched 

 and are frequently found to appear quite transparent. This appear- 

 ance is due to a watery looking fluid beneath the cuticular portion of 

 the body wall. 



Sometimes only the remnant of a larva (fig. 14; PI. II, vyuo) dead 

 of sacbrood is found in the cell. Such remnants vary in size. The 



Fig. 15.— Second stage: Dorsal view of an- 

 terior third of a larva dead of sacbrood. 

 (Original.) 



surface left from the removal of tissues 

 is somewhat roughened, indicating that 

 the removed portion has been taken 

 away piecemeal, and is more or less 

 transverse to the larva. 



Consistency of the larva in the first 

 stage. — The cuticular portion of the 

 body wall, which chiefly constitutes the 

 sac that characterizes the disease sac- 

 brood, is less easily broken at this time 

 than in the healthy larva. Wlien the 

 body wall is broken the tissues of the 

 larva, which constitute the contents of 

 the sac, flow out. This fluid tissue mass is less milky in appearance 

 than that from a normal larva. The granular (character of the con- 

 tents of the sac which is marked in later stages of decay is already in 

 evidence. By microscopic examination the granular appearance is 

 found to bo duo chiefly to fat cells. 



Condition of the virus in the first stage. — When larva? of this stage 

 uro crushed, suspended in sirup, and fed to healtliy bees, a largo 



Fio. 14.— First stage: Portion of a larva 

 dea^J of sacbroo*!, showing a more or less 

 tran-sveric roughened surface from wliich 

 the bees have removed a portion of tlie 

 Jarva ijiecemeal. (Original.) 



