44 BULLETIN- 431, V. S. DEPARTMENT OF AGRICULTTJEE. 



Table XI. — Resistance of sacbrood virus to putrefaction. 



Date of inoculation. 



Results of inoculation. 



Aug. 6, 1914.. 

 Aug. 7, 1914.. 

 Aug. 10, 1914. 

 July 20, 1915. 

 Sept. 13, 1915 

 Sept. 14, 1915 

 July 22, 1915. 

 Julys, 1915.. 

 May 22, 1915. 

 Sept. 22, 1915, 

 Aug. 18, 1915. 

 Sept. 16, 1914. 

 Sept. 25, 1914. 

 .Tuly 1,1915.. 



Sacbrood produced. 



Do. 



Do. 



Do. 



Do. 



Do. 



Do. 



Do. 



Do. 

 No disease produced. 



Do. 



Do. 



Do. 



Do. 



From Table XI it will be noted that the virus of sacbrood was 

 destroyed in the experiments recorded in from 7 to 10 days. As in 

 the case of fermentation, so in the case of putrefaction, it is to be 

 expected that the time for the destruction of the virus wiU vary 

 appreciably with the temperature at which the putrefactive processes 

 take place. 



RESISTANCE OF SACBROOD VIRUS TO CARBOLIC ACID. 



Larvae recently dead of sacbrood are crushed and strained. This 

 larval mass is diluted with carbolic acid in aqueous solution. About 

 10 parts of carbolic acid to 1 part of the larval mass is used. ■ This 

 suspension is distributed in test tubes and allowed to stand at room 

 temperature. Each tube contains the virus from about 15 larvae. 

 After periods, reckoned in days, colonies free from disease are inocu- 

 lated, each with the contents of a single tube added to sirup. 



Carbolic acid solutions of |, 1, 2, and 4 per cent were used in mak- 

 ing the suspensions. The following results were obtained from the 

 experiments : 



Table XII. — Resistance of sacbrood vinis to carbolic acid. 



Date of inoculation. 



Sept. 3, 1914. 

 Sept. 18, 1914 

 Sept. 3, 1914 . 

 Sept. 17, 1914 

 Aug. 12, 1915 

 Aug. 20, 1915 

 May 14, 1915. 

 Sept. 3, 1914 . 

 Sept. 18, 1914 

 June 23, 1915. 

 Sept. 17, 1915 

 Aug. 12, 1915 

 Aug. 21, 1915. 

 June 4, 1915.. 



Strength 



of car- 

 bolic acid 

 used. 



Per cent. 



Time in 

 suspen- 



Dnys. 

 1 

 16 

 24 

 38 

 50 

 50 



238 

 1 

 16 

 25 

 38 

 50 

 50 



251 



Results of inoculation. 



Sacbrood produced. 



Do. 



Do. 

 No disease produced. 



Do. 



Do. 



Do. 

 Sacbrood produced. 



Do. 



Do. 

 No disease produced. 



Do. 



Do. 



Do. 



