CHANGES IN FRESH BEEF DUKING COLD STORAGE. 15 



shaking the apparatus for 2 minutes each at the beginning, in the middle, 

 and at tlie end-of the reaction period. 



The results of this determination are reported in terms of percentages of 

 elementary nitrogen. 



Acidity was determined by titrating 100 c. c. of the extract against tenth- 

 normal sodium hydroxid, using phenolphthalein as an indicator. Results are 

 calculated in terms of lactic acid. 



Total soluble pJiosphorus. — For this determination, 100 c. c. of the 0.9 per 

 cent sodium chlorid extract was used. The method of determination was iden- 

 tical with the method used for the estimation of total phosphorus. 



Soluble inorganic pliospliorus was determined by the method of Chapin and 

 Powick (1915), which consists essentially in the removal of the protein mate- 

 rial by means of picric acid and the subsequent double precipitation of the 

 inorganic phosphorus from an aliquot of the filtered picric acid solution, first, 

 as magnesium ammonium phosphate and afterwards as ammonium phospho- 

 molybdate according to the method of Lorenz (1901). "Modification B" of 

 this method was used, no correction being made for the volume of the picric 

 acid coagulum. For the sake of brevity the details of this method must be 

 omitted. A complete description will be found in the original article by Lorenz. 



The results are reported in terms of percentages of elementary phosphorus. 



Soluble organic phospliorus. — The percentage of soluble organic phosphorus 

 was obtained by subtracting the percentage of soluble inorganic phosphorus 

 from that of total soluble phosphorus. 



co:mpositiox of differext muscle bundles from a hind quarter of 



A STEER. 



The original plan for the conduct of the autolysis experiment was 

 to determine the changes taking place in individual bundles of 

 muscles; but in practice it was found in the course of some unre- 

 ported work that it would be impracticable to obtain a sufficient 

 number of sterile samples by this procedure. This plan was there- 

 fore abandoned and it was decided to take the samples for incubation 

 at random from the muscular portions of the round of the hind 

 quarter, and then to determine the composition of the fresh material 

 by anal3'zing a composite sample taken from many parts of the round. 



It was recognized that there might be slight differences in the 

 composition of the different muscle bundles, a fact that would need 

 to be taken into consideration in interpreting the results of an autoly- 

 sis experiment conducted according to our plan. For this reason 

 the composition of five of the more important bundles of muscles 

 from the round of a fat .steer was determined. The quarter of beef 

 had been held in cold storage at 32°-34° F. for 48 hours previous to 

 disscfting out tlie muscle bundles, and .samples prepared for analysis 

 were held in cold storage for an additional period of 24 hours, 

 making a total of 72 hours' .storage before analytical work was 

 start<'(J. 



TaljJcs 1 U) •> inclusive; .siiow the roinixisition of (ivc muscle bundles 

 from the hind (juarter of the steer. 'J'abJes 1 and 3 show the com- 



