CHAN"GES IlSr FEESH BEEF DUEING COLD STOEAGE. 47 



ment being greater than the maximum increase that occurred in ex- 

 periment No. 1. These results are in conformity with those obtained 

 in the autolysis experiment. 



Slight apparent decreases, which for the present must be regarded 

 as due to possible inequalities in sampling, have taken place in the 

 total phosphorus. On account of the effect of the decreases in total 

 phosphorus upon the value of the other phosphorous compounds,, 

 changes in those constituents will be discussed in connection with 

 Table 25. 



Table 25 shows the distribution of nitrogen and phosphorus ex- 

 pressed as percentages of total nitrogen and total phosphorus. 



The distribution of the nitrogen compounds does not differ greatly 

 from that in case of experiment No. 1. There is an appreciable in- 

 crease in the proportion of total nitrogen present as soluble, non- 

 coagulable, proteose, and ammoniacal nitrogen, and a decrease in the 

 proportion present as coagulable nitrogen. 



Insoluble phosphorus shows appreciable decreases that range from 

 5.22 per cent in case of the rump to 8.58 per cent in case of the loin. 

 These results appear to be in conformity with the findings obtained 

 in the autolysis experiment, but in view of the results obtained in 

 other experiments of this series this seeming conformity must be 

 regarded as accidental. 



Total soluble phosphorus shows increases corresponding to the 

 decreases in insoluble phosphorus. 



Soluble inorganic phosphorus shows appreciable increases, Avhich 

 range from 10.37 per cent in the case of the round to 23.47 per cent 

 in the case of the loin. On the whole the increases in soluble inor- 

 ganic phosphorus are but slightly greater than similar changes in 

 this constituent in Experiment No. 1. On account of the much longer 

 storage period in Experiment No. 2 a larger increase in inorganic 

 pho.sphorus might have been expected; but in this connection it is; 

 interesting to note that the material used for this experiment already 

 contained a considerably higlier percentage of preformed inorganic 

 phosphorus than did the material used in the first experiment. It 

 would appear as though the larger quantity of inorganic phosphorus 

 present in the material used in the second experiment either of itself 

 retarded the rate of change of organic phosphorus into inorganic 

 forms or was indicative of some retarding agency. 



Interof^ting liglit is thrown on this (jticstion by the i-esults of tho 

 autolysis ex|K'rinient, as shown in Table 11, where under the head- 

 ing " Inorganic phosphorus " it may be noted that the increase in 

 this constituent takes plnce most rapidly during the first 7 days 

 of the (;x])eriment. Thus during the first 7 days the relative in- 

 crea.se amoimts to 52.78 per cent, while during the total incubation 

 jM'iiod of 100 fhiys the iclativc iucreast; amounts to oidy 05.27 per 



