64 BULLETIN 433, U. S. DEPARTMENT OF AGEICULTUEE. 



the storage period, and when these changes are expressed in terms 

 of percentages of the initial ratio of inorganic to total phosphorus 

 the changes are smaller, on the whole, than those obtained in Ex- 

 periment No. 1, where the storage period was but two weeks. In 

 view of the unusually large proportion of inorganic phosphorus 

 contained in the original material large increases were not to have 

 been expected, and the changes that did take place should be viewed 

 from the standpoint indicated in the discussion of the inorganic 

 phosphorus results obtained in Experiment No. 2, where a some- 

 what similar condition prevailed. As the material used in this ex- 

 periment was not quantitatively comparable with that used in any 

 of the earlier experiments as regards the amount of performed in- 

 organic phosphorus that it contained, there is no criterion by which 

 to judge the quantitative significance of the changes in inorganic 

 phosphorus in the present experiment. 



The increments in the ratios of soluble organic to total phosphorus 

 are small in comparison with the length of the storage period, though 

 they constitute a decidedly high percentage of the initial ratios. 

 Relations of the same nature have already been pointed out and dis- 

 cussed in connection with Experiment No. 2. For the rest, the 

 changes in the soluble organic phosphorus have no more significance 

 than the corresponding changes in the soluble inorganic phosphorus. 



EXPERIMEXT NO. 5. 

 HISTOBY OF CAKCASS. 



A "grade" Shorthorn steer 3 years old, of prime quality and 

 highly finished, was slaughtered by the usual methods and the car- 

 cass was allowed to hang 1 hour and 15 minutes on the killing floor 

 before being run into the fore cooler. The warm carcass weighed 

 860 pounds. The carcass was held 19 hours in the fore cooler and 

 21 hours in the main cooler. The hind quarters were then cut from 

 the carcass, carefully wrapped, and transported to the city whole- 

 sale market of the packing house, where they were held five hours in 

 a cold-storage room having a temperature of about 38° F., and were 

 then transported to the bureau's cold-storage rooms. 



STOEAGE. 



The beef was unwrapped, weighed, and hung in cold-storage room 

 No. 1 until the next day, when one quarter was prepared for analysis. 

 The second quarter was held in cold storage for an additional period 

 of 74 days. 



The temperature of the cold-storage room ranged for the most part 

 between 34° and 38° F. On each of two occasions, however, the 

 temperatuie ran up to 41° F., and on one occasion it rose to 50° F. 

 ior a part of a day in consequence of difficulties with the refrigerat- 



