70 BULLETIN" 433, U. S. DEPARTMENT OF AGEICULTUEE. 



Fairly marked increases in the amount of total soluble nitrogen 

 present in the meat have occurred during this storage period. These 

 are the first appreciable increases in total soluble nitrogen that have 

 taken place in any of the carcasses examined thus far. These data 

 are in keeping with the previously noted increases in total solids in 

 this experiment. 



Coagulable nitrogen shows appreciable decreases, which are prac- 

 tically the same as those noted in the previous experiments. As has 

 been previously noted, the data for coaguable nitrogen show merely 

 the variations in the actual reserve amount of this constituent, and 

 do not indicate the true extent of the transformation of coagulable 

 proteins into noncoagulable forms, inasmuch as the supply of coagu- 

 lable protein is being replenished from the insoluble protein at the 

 same time as the coagulable protein is being transformed into non- 

 coagulable compounds. The true extent of the change of coagulable 

 protein into noncoagulable forms is shown in the data for noncoagu- 

 lable nitrogen. 



Increases in noncoagulable nitrogen, which range from 29.12 to 

 40.45 per cent, are much greater than the increases that took place 

 in this constituent in the previous experiment. 



Changes in proteose nitrogen are in the nature of increases, which 

 are greater than those that took place in any of the previous experi- 

 ments except Experiment No. 2. 



The amino nitrogen almost doubled in the round and rump, and 

 more than doubled in the loin during the storage period. The in- 

 creases are larger than any corresponding increases that occurred in 

 this constituent during the shorter periods of the previous experi- 

 ments. The results are in continued conformity with the results of 

 the autolysis experiment. 



Ammoniacal nitrogen increased in each of the cuts analyzed. The 

 increases in the round and rump, however, were not as great as the 

 corresponding increases in Experiment No. 4, a fact which stands in 

 no connection with the amounts of preformed ammonia in the ma- 

 terial, but which must be accounted as a distinct exception to the 

 rule that seems to have api^lied in most of these experiments. The 

 increase in the loin, on the other hand, was tKe largest that had yet 

 been observed in this constituent. 



Changes in total phosphorus consisted in quite marked apparent 

 decreases, the significance of which is not clear. 



Table 46 shows the distribution of nitrogen and phosphorus ex- 

 pressed as percentages of total nitrogen and total phosphorus. 



Soluble nitrogen makes up a smaller proportion of the total nitro- 

 gen of the fresh quarter of this carcass than it made in case of any 

 fresh quarter previously examined. 



Coagulable nitrogen forms a smaller proportion of the total nitro- 

 gen of the meat, both at the beginning and at the end of the storage 



