86 BULLETIlSr 433, U. S. DEPARTMENT OF AGEICULTUEE. 



carcass had been held in storage for a period of 1T7 days, are only 

 slightly larger than those that took place in Experiment No. 5, where 

 the carcass had been held in storage for a period of only 74 days. 



The increases that have taken place in proteose nitrogen are com- 

 paratively small, being only slightly gi-eater than those which took 

 place in the carcass stored two weeks, and not so large as those which 

 took place in the carcass stored four weeks. 



Amino nitrogen more than doubled during the 177-day storage 

 period in each part of the carcass examined. That the largest in- 

 creases were effected during the longest period of storage is in final 

 conformity with the results obtained in the autolysis experiment. 



Ammoniacal nitrogen increased to a greater degree during the 

 storage period in this experiment than it had previously increased 

 during the shorter storage periods, the increases each amounting to 

 approximately 100 per cent. 



The total phosphorus content of each portion of the stored quar- 

 ter was less than that of corresponding portions of the fresh quarter. 

 The average apparent decrease was greater than the average ap- 

 parent decrease in this constituent in anj of the previous experi- 

 ments, and was accompanied by the largest average loss of total 

 nitrogen. The cause of these apparent losses is not evident. 



Table 60 shows the distribution of nitrogen and phosphorus ex- 

 pressed as percentages of total nitrogen and total phosphorus. These 

 data are of particular interest in view of the fact that one of the 

 quarters of this carcass of beef had been held in cold storage at a 

 temperature above freezing for a period of nearly six months. 



As regards the nitrogen compounds, the quarter of beef that had 

 been held in cold storage for a period of 180 days contained a larger 

 proportion of its total nitrogen in the forms of total soluble, non- 

 coagulable, amino, and ammoniacal nitrogen, respectively, than did 

 any of the carcasses that had been stored for shorter periods of time. 

 On the other hand, the proportions of total nitrogen present in the 

 forms of proteose and coagulable nitrogen had, in this quarter, val- 

 ues that were intermediate between their highest and their lowest 

 values in this series of experiments. 



Insoluble phosphorus, even with the long storage period of this 

 experiment, showed irregular changes, increasing in the loin and de- 

 creasing in the round and rump, so that no additional light is thrown 

 upon the nature of the changes in this constituent during cold storage. 



Changes in total soluble phosphorus are irregular and have no 

 determined significance. 



The actual increases which occurred in the ratios of soluble inor- 

 ganic phosphorus to total phosphorus during the storage period of 

 this experiment are greater than the corresponding increases which 

 have taken place during the shorter periods of previous experiments, 

 although the change per cent in this ratio, in case of the round, is 



