BACTERIA IN COMMERCIAL BOTTLED WATERS. 5 



Of 57 foreign springs (see Table V) — 



29 (51 per cent) contained no B. coli in 10 cc quantities. 

 28 (49 per cent) contained B. coli in 10 cc quantities. 

 25 (45 per cent) contained B. coli in 5 cc quantities. 

 21 (37 per cent) contained B. coli in 1 cc quantities.-" 

 16 (28 per cent) contained B. coli in 0.1 cc quantities. 



8 (14 per cent) contained B. coli in 0.01 cc quantities. 



2 (3 per cent) contained B. coli in 0.001 cc quantities. ^ 



Forty (70 per cent) gave counts of less than 100 on one or more 

 bottles after incubation for two days at 37° C. _ . 



Twenty-five (44 per cent) gave average counts of less than 100 

 per cc at 37° C. 



The highest count shown at 37° C. was 37,000 per cc. This sample 

 gave an average count of 16,000 per cc, and B. coli were found in one- 

 third of the bottles examined in 5 cc quantities. 



Two imported waters bearing on their labels the words "bacterio- 

 logically pure" gave the following results: 



Sample No. 1; six bottles examined — 



Lowest number of organisms per cc developing on gelatin at 20° C 700 



Average number of organisms per cc developing on gelatin at 20° 2, 450 



Lowest number or organisms per cc developing on agar at 37° C 300 



Average number of organisms per cc developing on agar at 37° C 1, 250 



4 bottles contained B. coli in 10 cc quantities. 

 4 bottles contained B. coli in 5 cc quantities. 



4 bottles contained B. coli in 1 cc quantities. 



2 bottles contained B. coli in 0.1 cc quantities. 



Sample No. 2; seven bottles examined— 



Lowest number of organisms per cc developing on gelatin at 20° 120 



Average number of organisms per cc developing on gelatin at 20° C 9, 410 



Lowest number of organisms per cc developing on agar at 37° C 40 



Average number of organisms per cc developing on agar at 37° C 482 



6 bottles contained B. coli in 10 cc quantities. 



5 bottles contained B. coli in 5 cc. quantities. 

 5 bottles contained B. coli in 1 cc quantities. 

 5 bottles contained B. coli in 0.1 cc quantities. 



3 bottles contained B. coli in 0.01 cc quantities. 



Among the organisms which have been isolated from the above 

 samples are: B. coli, B. cloacse, B. mycoides, B. paratyphosus B, B. 

 aerogenes, B. aurantiacus, M. citreus, B. maritimum, B. ovale, B. pro- 

 digiosus, B. Jiuorescens liguefaciens, B. Jluorescens norir-liquefaciens, 

 B. subtilis, and long-chain streptococci. 



Molds of the genera Trichoderma, Penicillium, Cladosporium, 

 Citromyces, Fusarium, Actinomyces, and Sporotrichum were identi- 



1 Any potable water supply containing 5. coli in 1 cc quantities is considered suspicious by health 

 departments and is at once investigated. 



2 Water containing B. coli in 0.001 cc quantities is too suggestive of dilute sewage to be accepted by 

 anyone. 



