BACTERIA IN COMMEECIAL BOTTLED WATEES. 7 



The results clearly show that bottled waters can be made to con- 

 form to the requirements of the United States Public Health Service 

 for drinking water furnished upon trains; that is, that not more than 

 one 10 cc sample out of five should show the presence of B. coli. 



TABULATED DATA. 



Table I. — Results of the bacteriological examination ofivater collected from spring No. 1. 





Colonies of organisms per cc de- 

 veloping after— 



Smallest quantity 

 were found- 



in which 



Description of sample. 



2 days' in- 

 cubation 

 on nutrient 

 agar at 

 37° C. 



4 days' incubation on 

 nutrient gelatin at 

 20° C. 



B. coli. 



Molds. 





Total 

 coimt. 



Liquefl- 

 ers. 



At time 

 of collec- 

 tion. 



2 days 

 after col- 

 lection. 



"Clean" bottle rinsed with 100 cc ster- 



1,000,000 

 900,000 



700,000 



1,000,000 



4,800 



790,000 



840.000 



3.000 



4^500 



410,000 



1,400,000 

 540,000 



1,100,000 



1,400,000 



700 



400,000 



1,000,000 



48,000 



38,000 



900,000 



17,000 

 800 



120,000 



59,000 



18 



18, 000 



90,000 



1,000 



1,000 



10,000 



cc. 



0.1 



.1 



.01 

 .01 

 1.0 



.1 



.1 

 (') 

 (0 



.01 



.01 



.001 



2.001 



cc. 



0.01 



1.0 



.001 

 .001 

 5.0 



.01 

 .1 



(1) 



(0 



.001 



cc. 



Do 





"Dirty" bottle rinsed with 100 cc ster- 





Do 





16 caps rinsed with 70 cc sterile water . . 

 Water used for washing and rinsing 



" 





Do 







001 



Do 



.001 



Water from creek 100 feet from bot- 





Water from swimming pool, after use 





Water from swimming pool, after use 













12 bottles collected after Inspection; 



126,000 



152, 400 



5, 150 





.001 









1 No B. coli were present in 10 cc quantities. 



2 This determination was made at the time the sample was received at the laboratory. 



Table II. — Results of the bacteriological examination ofivater collected from spring No. 2. 



Description of sample. 



"Clean" bottle rinsed with 100 cc ster- 

 ile water. . . ; 



Do -.. 



12 caps rinsed with 100 cc sterile water. . 



Water from bottling spring 



Do 



Water from creek 5 feet from bottling 



spring 



Do 



10 bottles collected after inspection; 

 average results , 



Colonies of organisms per cc de- 

 veloping after — 



2 days' in- 

 cubation 

 on nutrient 

 agar at 

 37° C. 



280,000 

 300,000 

 870 

 137,000 

 117,000 



310,000 

 297,000 



2,220 



4 days' incubation on 

 nutrient gelatin at 

 20° C. 



Total 

 count. 



800,000 



500,000 



1,100 



110,000 



85,000 



G) 

 (1) 



2,262 



Liquefi- 

 ers. 



800 



33,000 



100 



2,000 



1,100 



Smallest quantity in which 

 were foimd — 



At time 

 of collec- 

 tion. 



0.1 

 1.0 

 5.0 

 1.0 

 1.0 



.001 

 .001 



2.1 



2 days 

 after col- 

 lection. 



1.0 

 1.0 

 5.0 

 .1 

 .1 



.0001 

 .0001 



Molds. 



1 Liquefied. 2 This determination was made at the time the sample was received at the laboratory. 



