68 



BULLETIN 846, U. S. DEPARTMENT OF AGRICULTURE. 



The divisor in 



was de- 



manner 



5,000,000 bacteria, with a penalty of more than unity if more than 

 5,000,000 bacteria were present and vice versa. In the chart (fig. 4) 

 there are 8 dots above the 5,000,000 line and 3 crosses below it. 

 The penalty imposed in the case of samples with dots above this line 

 is not sufficiently great to cause any of them to have a formula value 

 greater than zero. The choice of 5,000,000 is further vindicated 

 by the extensive experiences of three members of the Bureau of Chem- 

 istry in examining commercial frozen egg products and by the 

 narrated experiences of a bacteriologist in a commercial egg-breaking 

 plant who stated that in his opinion no frozen egg product should 



contain more than 5,000,000 bac- 

 teria at any season of the year. 

 X 

 500,000 

 termined hi a similar 

 (fig. 5). 



The values assigned to Y and 

 Z are in conformity with what 

 juries and judges have been found 

 to tolerate or condemn in the 

 past, and are also in accord with 

 the 5 per cent bad-egg ruling of 

 the Department of Agriculture. 



For sugared yolk, which may 

 be recognized by the fact that 

 the solids not fat will be almost 

 double the ether extract, as com- 

 pared with approximately equal 

 amounts of these two constituents 

 in the case of unsugared yolk, this formula must be modified to 

 the following form, based upon the plots: 



§ 



> 

 ) 



; $ j 



\ > 



. 



i > 



: 











*! 



i Q 



/S,O0O,O0O 



/4,000,00O 



/3,000.00O 



X /2,000,000 



k//,ooaooo 



tv /0.O00000 

 & £,000,000 

 y 3,000.000 

 & 7,000.000 

 R 6.OOO.OO0 













> 

























> 



: 































_ 













































> 



« 













































j 



r 





















, 



t 











































































































^ 4,000,000 

 ^ 3,000,000 



^ zpoo.ooo 



^ AOOO.OOO 

 n 









> 



c 









































I 



















< 



1 



! 















\ ' 

















Fig. 4.— Total bacterial counts. 



10.0 CR- 0.205$ -0.5) + 10.0 (S-0.0Q8Q- 1.158) -25.0 ( £7+0.029 T 



-°- 798) + v+ 57i^ + 5oim + Y+Z =° 



It is important to see how these formulas actually work out in 

 practice. At the bottom of Tables 1 to 23 will be found the formula 

 values for the samples, the formula being calculated in each case for 

 the average of the results of the individual analysts. In Tables 1, 

 2, and 3 the formula values are negative, and of a very appreciable 

 magnitude. In Table 4 the formula value is small, as would be 

 expected in all samples of egg white. In Table 5 all the values are 

 negative, and decrease in magnitude with the length of time the eggs 

 were held in storage. It will be noted that in the first three formula 

 values there are terminal plus signs. These indicate that it probably 



