4 BULLETIN 1222, U. S. DEPARTMENT OF AGRICULTURE. 



time of sealing, so that it seems probable that these figures are 

 somewhat too high throughout. At the second weighing (June 30) 

 the larva? were seen to differ greatly in size, ranging from 2.3 milli- 

 grams to 14.3 milligrams. The largest in the lot was some distance 

 from the margin of the frame near a patch of brood about 4 days 

 old. On July 1, larvae 3 days old also displayed great differences in 

 size. Three larvae from the end of the frame, close to the brood- 

 nest, were larger than the other two and weighed 54.4+ milligrams 

 each. The two smaller, from the lower edge of the frame, weighed 

 only 12.6 milligrams each. These smaller larva? were still being fed 

 larval jelly, while the three larger ones were being fed honey and 

 pollen. On July 2, great differences in size were still evident, even 

 between larva? in adjacent cells. Two larva? were beginning to be 

 capped, one of these weighing 164.1 milligrams. The smallest larva 

 in the lot weighed only 77 milligrams. On July 3, all larva? of this 

 lot were capped except one, which was partly capped. Capped larva? 

 of this and the three succeeding lots were not weighed. Weighings 

 of 15 mature larva? from this colony during the same season gave 

 a total weight of 2,374.7 milligrams and an average of 158.31 milli- 

 grams. 



For comparison in the table these weights are used for larva? 4£ 

 to 5 days old for Lots 2 to 4, and for larva? 5| to 6 days old for 

 Lot 5. 



Lot 3 (June 29). — About 40 cells containing newly hatched larva? 

 were selected from the same frame as Lot 2. At the first weighing 

 (June 30) the larva? appear quite uniform in size. At the second 

 weighing (July 1) the weights ranged from 3.3 to 15.5 milligrams, 

 and at the third weighing from 13.1 to 30.3 milligrams. On 

 July 3, some cells of this series were completely sealed, one or two 

 were partly sealed, but most of them were still open. Near the 

 bottom of the frame there were three cells of this lot in a row ; the 

 two end cells were sealed, while the middle one was still open. The 

 two sealed larva? weighed 158.5 milligrams each ; the one in the open 

 cell weighed 117 milligrams. Another larva from an open cell 

 weighed only 90.5 milligrams while a fifth weighed 114.3 milli- 

 grams. All the cells in this lot were sealed on July 4. 



Lot If. (July 5). — About 50 cells on the same frame were marked, 

 great care being taken to mark only those cells containing larva? of 

 minimum size. On July 6, 5 larva? weighed 3.05 milligrams, an 

 average of 0.61 milligram. On the opposite side of the frame near 

 a patch of brood were much larger larva? belonging to this lot, one 

 of the largest of these weighing 2.2 milligrams. On July 7 the larva? 

 in marked cells continued to show considerable differences in size. 

 The larva? of the group isolated among cells containing eggs re- 

 mained smaller than those on the opposite side of the comb, located 

 next to older larva?. Most of the larva? at this time covered a circle 

 one-half to two-thirds of the diameter of the bottom of the cell. The 

 total weight of five larva? taken at random from both sides of the 

 comb was 31.75 milligrams, an average of 6.35 milligrams. The in- 

 dividual weights, in milligrams, were as follows: 2.85, 3.7, 3.7. 9.55, 

 11.95. All larva? were still fed with larval jelly. On July 8, the 

 weights of 5 larva? ranged from 17.5 to 55.3 milligrams. The 

 larger were uniformily near unsealed brood, the smaller among eggs 

 near the middle of the frame. The smaller larva? occupied less than 



