14 



BULLETIN 1233, U. S. DEPARTMENT OF AGRICULTURE. 



growing near the nurses' quarters at Ancon Hospital, Ancon, Canal 

 Zone, and transplanted to this location about three years before, was 

 inoculated with live nemas (Aphelenchus cocophilus) January 10, 

 1922, by Dr. N. A. Cobb, with the assistance of Mr. Zetek. Since 

 Doctor Cobb left a few days later, the observations on the progress of 



Fig. 1. — Diagram to show general shape and size of trunk of coconut palm shown in 

 Plate VII, its relation to ground level, and location of four sections that were cut 

 and photographed. Location of injury through which termites entered is also shown. 



the disease were made by Mr. Zetek, assisted by Mr. Molino. The 

 inoculation was made under aseptic conditions into two petioles and 

 live nemas, free in a little water, were used. When transplanted 

 the tree was placed too deep in the ground, but it was growing very 

 well. 



