30 BULLETIN TOA, U. S. DEPARTMENT OF AGRICULTURE. 
been found to reappear in deficient numbers in the perjugate genera- 
tion of crosses with horny and with sweet varieties of maize. 
By adding all of the ears expected to have 25 per cent of the seeds 
waxy, including the 45 ears previously reported (7), there are 198 
ears having 102,427 seeds. The mean percentage of waxy seeds is 
93.9 and the deviation of 1.1 per cent would be expected to occur as 
the result of chance but once in 10 raised to the fifteenth power. « 
This deviation though apparently small is certainly too large to be 
attributed to chance. The apparently slight deviation from 25 per 
cent and the large number of individuals necessary to establish the 
significance of such small deviations suggest the possibility that such 
departures may not be uncommon for other character pairs which 
have not been subjected to such an exhaustive test. 
Although the observed deviation could not reasonably be expected 
to occur as the result of chance, all of the individuals do not approxi- 
mate the mean percentage of the whole. An analysis of the “ good- 
ness of fit” of the individual ears to their observed mean by the use 
of the method proposed by Yule (16) showed that the individual 
plants did not form a single homogeneous group with a mean per- 
centage of waxy seeds below 25 per cent, but that many of the in- 
dividual ears could not be considered as merely chance deviations. 
Thus in hybrids between waxy and horny individuals the mean 
percentage of waxy seeds reappearing in the perjugate generation 
is below the expected 25 per cent by an amount too large to be due 
to chance. The differences between individual plants with respect to 
this character are also too large to be due to chance. 
Reciprocal crosses clearly indicated that in some cases the per- 
centage of male gametes bearing the waxy character was below that 
expected. It has not been possible thus far to determine whether 
this observed deficiency is due to a higher death rate, reduced vigor, 
-or a failure of equal segregation. 
The deficiency of waxy seeds can not be due to a fractionation of 
this character, since such a fractionation could not result in horny 
seeds but should give seeds that were neither horny nor waxy. 
INHERITANCE OF ALEURONE COLOR. 
The crosses made between the white waxy Chinese variety and 
the colored pop variety also produced excellent material .for the 
study of aleurone color. The results in a very striking manner con- , 
form to the Mendelian proportions expected in monohybrid and 
dihybrid ratios. 
In many crosses involving aleurone color the inheritance is blended 
rather than alternative, and classification is more or less arbitrary. 
In the Algeria & Chinese hybrids the classes were exceptionally good, 
