64 BULLETIN 754, U. S. DEPARTMENT OF AGRICULTURE. 
to have equal numbers of white and colored seeds, was of necessity 
confined to one parent, it became of interest to know whether that 
parent was defaulting equally in the number of male and female 
gametes carrying white aleurone. The progeny of ear No. 1099 had 
6,804 seeds born on homozygous white plants, but pollinated with 
pollen from heterozygous colored plants. The percentage of these 
white seeds was 47.5. The heterozygous colored plants grown from 
seeds of ear No. 1099 bore 7,182 seeds that were the result of pollen 
from the homozygous white plants. The percentage of these white 
seeds was 49.6. 
The difference between the white plants pollinated with pollen 
from the colored plants and the colored plants pollinated with pollen 
from the white plants is 2.1+0.571 per cent. This difference is 
3.67 times the probable error and would seem to indicate that the 
heterozygous colored plants were making the expected proportions of 
female gametes with the observed shortage occurring in the male 
gametes. 
A similar analysis of the progeny of ear No. 1111 shows the per- 
centage of male gametes bearing white aleurone to be below the 
expected by 5.2 times the probable error. 
The homozygous white plants of ear No. 1129 bore 7,434 seeds 
that were the result of pollen from heterozygous colored plants of 
this same progeny. The observed percentage of white seeds is 49.7. 
The heterozygous colored plants bore 3,493 seeds that were the result 
of pollen from the homozygous white plants. The observed percent- 
age was 50. The difference between these two groups is but 0.3 per 
cent and can not be considered significant, though the variation is 
in the same direction as the cases previously considered. 
The progeny of ear No. 1130, progeny of the hybrid Dh 287, varied 
slightly in the other direction, the male parents proving to have a 
higher percentage of white than of colored gametes, and while the 
white female gametes also were in excess, the excess of white male 
gametes exceeded-that of the white female gametes by 0.5 per cent, 
which is not significant. 
Combining the progeny considered above, there were 26,174 seeds 
which were borne on homozygous white plants, but the result of pol- 
len from heterozygous colored plants. The observed proportion of 
white seeds in these was 48.8 per cent. 
The heterozygous colored plants which were the result of pollen 
from homozygous white plants bore 21,698 seeds. The observed per- 
centage of white seeds in these is 50. The difference between these 
two groups is 1.2+0.31 per cent. This difference is 3.87 times the 
probable error, which is a rather large difference to be ascribed to 
chance. 
