84 BULLETIN 754, U. S. DEPARTMENT OF AGRICULTURE. 
The colored horny combination in the male gametes was in excess 
by 17 and deficient in the female gametes by 21; so a deficiency of 4 
would be expected on the self-pollinated ear, and we find a defi- 
ciency of 8. 
The colored waxy combination in the male gametes is deficient by 
18; in the female gametes this same combination is in excess by 8; 
so a deficiency of 10 would be expected in the self-pollinated ear, 
and a deficiency of 18 is noted. 
The white horny combination is deficient by 17 in the male 
gametes and in excess by 17 in the female gametes; so that they 
would be expected in the theoretical proportion on the self-pollinated 
ear, and we find an excess of 6. This agreement of evidence derived 
from so many different sources would indicate that the departures 
observed were the result of a definite and unexpected gametic series 
formed by the plant which bore ears Nos. 1799 and 1800, rather than 
fluctuations due to chance. 
The three ears that were obtained when plants grown from white 
waxy seeds were used as the male parent had a total of 1,293 seeds 
with a correlation of 0.610+0.025, but since one ear of the three 
has been shown to be of entirely different coupling this ear should 
not be included in the totals. Omitting, then, this one ear, No. 
1795, which is outstanding and does not belong to the series, the 
total number of seeds then becomes 740, with a correlation of 
0.737+0.025—a deviation from the expected 0.8 of but 2.5 times the 
probable error. 
Nine ears were secured from plants grown from the colored waxy 
seeds as the male parent, and two ears were secured from plants 
grown from the white horny seeds as the male. These ears are also 
shown in Table XXXVI. The correlation for the total number of 
seeds is not significant. One ear, however, No. 1781, has a negative 
association between C’ and H of 0.220+0.059, a deviation from a 
zero correlation by 3.7 times the probable error. This ear could in all 
probability be looked upon as the result of chance fluctuation in 
sampling, though closely approximating the 0.27 expected when a 
plant heterozygous for the color factors C and # and horny 
endosperm texture with a 3-1-1-8 coupling between the factor C and 
waxy endosperm is crossed with a homozygous waxy plant heter- 
ozygous for the two factors for color. 
As a test of the possibilities of securing a correlation when cross- 
ing two plants, one heterozygous for endosperm texture and without 
the factor for color C, the other homozygous for endosperm texture 
and heterozygous for the factor (, crosses were made between plants 
from white horny seeds and plants from colored waxy seeds. ‘These 
crosses were made, using the colored waxy plants as the female parent 
and also as the male parent. 
