LEAF-SPOT OF ALFALFA AND RED CLOVER. 19 
as this. The red-brown color of the Pseudopeziza stroma produced 
by this medium on these white blocks (Pl. I, B) could be recognized 
at an earlier date than the pale-colored growth which developed 
when other media were tried. 
Spores of Pseudopeziza trifolii were tested in the same way with 
those of P. medicaginis, though not as extensively. The results are 
given in Table VI. 
TABLE VI.—Viability test of ascospores of Pseudopeziza medicaginis and Pseu- 
dopeziza trifolit viable when dried on plaster of Paris blocks in the laboratory 
and out of doors. 
Place and date of ending Time Place and date of ending Time 
desiccation. (days). | Results. desiccation. (days), | Results. 
PSEUDOPEZIZA MEDICAGINIS. PSEUDOPEZIZA MEDICAGINIS— 
continued. 
In Iaboratory: 
inept bees eee 32 + Out of doors—Continued. 
Panevoo M0152 os 2e ce es: 36 4- May 20, 1916...-.......-.-. 120 a 
1D EEE S} TRG ee AOE eee . 37 + INO, Ws, WONG. coca ko suecs 108 + 
mebesle 19th ee. 2 Sore Pes s 4 2 38 + APTS CG FIOIG Sse eke ease 159 ae 
eereiO1G. 2 se! 39| + Nay 20NIGI5 nmi em 212| 0 
WeewIGT19ID 2 ek = sate 48 + Septic] s191G eae eee 303 + 
ing, 2 Ga ene eee 49 + DO gaetoacicne se Gage sees 329 0 
Mar 2651915253 522552325522 63 ft 
AT a a sae eee 66 0 PSEUDOPEZIZA TRIFOLII. 
Jan. 12, 1916 es 72 ae 
Jan. 17, 1916 76 + In laboratory: 
Jan, 23, 1915. 76 + Jan. 17, 1916... 36 + 
Jan, 22, 1915 79 + Mar. 7, 1916... 63 + 
Jan. 1, 1915. 85 0 Feb. 15, 1915... 64 + 
Aug. 26, 1915 a 93 0 Apr. 6, 1916... 112 0 
Wosns 22525k a2 94 0 May 15, 1916... 113 + 
i \aypeth a) Gee eee ee 100 0 Apr. 20, 1916 120 0 
Hebi; 191682 24.222...) 104 + Sept. 1, 1916 258 0 
Std LD es eSeepeeeee 210 0 DD Ormsa eee ciecion sowie 256 0 
TET Gy Ee Se es ee 357 0 Out of doors: 
Out of doors: denny Ws WUG. sceoebouededks 28 + 
Weeq9 91S. Je saeco dese: 27 + OL aos se eg ss ee 25 + 
WECMIG T1919 bo ook eee 34 + Weng ey TONG ese sae aue5e 104 aa 
Dec. 22, 1915......- Le = cil 52 0 Apr 106s see Pee wee 107 0 
Part 0(G2 es ast Se. 77 ate Sion, 2 WIGS so Leosgs shaved 265 0 
Janet, 1916-032): 2. ae eee 78 + Septic W916 casas 265 0 
MOUs 10 1916-2 cceee ses. see 106} + 
From these results it appears that drying alone can not be ‘de- 
pended upon to kill all the spores of either Pseudopeziza in less than 
one year. Severe freezing during drying had no apparent effect. 
If conditions for survival are as favorable on the seed as on plaster of 
Paris blocks, the spores should be able to live from one season to the 
next on the seed. But unless conditions on the seed are more favor- 
able than on the block, they should not be able to survive and 
germinate during a second year. 
PATHOGENICITY OF THE FUNGI. 
METHOD OF MAKING INOCULATIONS. 
In all inoculations that have been made, ascospores alone have been 
used as the inoculum. The conidiumlike structures which have 
been described are produced almost wholly in the substrate, and 
since only rarely will one of them separate from the mycelium, no 
