18 



BULLETIN 810, U. S. DEPARTMENT OF AGRICULTURE. 



fection results the germ is considered as having been destroyed. 

 Observations have been made on the virus kept at incubator, room, 

 outdoor, and refrigerator temperatures and shielded from the light 

 in each instance. The experiments conducted with Bacillus pluton 

 in these environments are summarized in Tables II, III, IV, and 

 V which follow : 



Table II. — Resistance of Bacillus plnton to drying at incubator temperature 



July 3 : . 

 July 10.. 

 July 17., 

 July 25.. 

 Aug. 3... 

 Aug. 15.. 

 Sept. 1 . . 

 Sept. 16. 

 Sept. 29. 



June 29.. 

 July 13.. 

 July 9... 

 Sept. 20. 

 Sept. 17. 



Sept. 8. 



Date of inoculation. 



1914. 



1916. 



Period of exposure. 



Months. 



24 



Days. 

 10 

 17 

 24 



2 



7 

 17 





 11 







Results of inoculation. 



European foulbrood produced. 

 Do. 

 Do. 

 Do. 

 Do. 

 Do. 

 Do. 

 Do. 

 Do. 



Do. 

 Do. 

 No disease produced. 

 Do. 

 Do. 



Do. 



Table III. — Resistance of Bacillus pluton to drying at room temperature 



Date of inoculation. 



Period of drying. 



Months. 



Days. 



1 



1 



1 



21 



2 



8 



2 



21 



3 







3 



14 



9 



10 



12 



6 



11 



13 



11 



15 



11 



18 



12 



2 



14 



10 



14 



18 



24 







36 







Results of inoculation. 



July 25, 1914. 

 Sept. 18, 1914 

 Sept. 1, 1914. 

 Sept. 28, 1914 

 Sept. 29, 1914 

 Oct. 6, 1914.. 

 Jan. 29, 1915. 

 July 9, 1915. . 

 Aug. 3, 1914.. 

 June 22, 1915. 

 June 25, 1915. 

 Aug. 9, 1915.. 

 Sept. 17, 1915 

 Sept. 20, 1915 

 Sept. 8, 1916. 

 Do 



European foulbrood produced. 



Do. 



Do. 



Do. 



Do. 



Do. 



Do. 



Do. 

 No disease produced. 



Do. 



Do. 



Do. 



Do. 



Do. 



Do. 



Do. 



Table IV. — Resistance of Bacillus pluton to drying at outdoor temperature 



Date of inoculation. 



Period of drying. 



Results of inoculation. 



Sept. 2, 1914 . . 

 Sept. 16, 1914. 

 Oct. 13, 1914.. 

 May 26, 1915. . 

 June 19, 1915.. 

 May 17, 1915. . 

 Aug. 3, 1915... 

 Aug. 17, 1915., 

 June 23, 1916.. 



Months. 



Days. 

 33 

 47 

 74 

 21 

 18 

 17 

 3 

 17 



European foulbrood produced. 



Do. 



Do. 



Do. 



Do. 



Do. 

 No disease produced. 



Do. 



Do. 



