EUROPEAN FOULBROOD. 



23 



lively. In Tables XI and XII. which follow, are summarized the 

 experiments performed : 



Table XI. — BaciUus pliiton in the presence of putrc(actire proccHSCs at incubator 



temperature 



nateof inocululion. 



JimoSn, 191fi. 

 Sept. 2, 1916. 

 Aug. 1."), 1916. 

 Sept. 7, 1915. 

 Jiily5, 191(i.. 

 Sept. 10, 1915 

 Julys, 1910.. 

 Aug. 23, 1915. 

 Aug. 30, 1915. 



Period of 





putre- 



Results of inoculation. 



faction. 





Days. 

 9 



European foulbrood produced. 



7 



No disease produced. 



8 



Do. 



13 



Do. 



15 



Do. 



16 



Do. 



18 



Do. 



19 



Do. 



26 



Do. 



Taiile XII. — Bdcillu-'i pliiton hi the presence of putrefactive processes at room 



temperature 



Date of inoculation. 



Period of 

 putrefac- 

 tion. 



Results of inoculation. 



Aug. 4, 1914. 

 July 17, 1915. 

 July 5,1916-. 

 July 23, 1915. 

 Aug. 14, 1914. 

 Sept. 17, 1915 

 Aug. 25, 1916. 

 Sept. 2, 1916. 

 Aug. 3, 1915.. 

 Aug. 28, 1916. 

 Sept. 1,1914. 

 Sept. 16, 1914 

 Aug. 12, 1916. 



Days. 



European foulbrood produced. 



Do. 



Do. 



Do. 



Do. 



Do. 



Do. 



Do. 



Do. 

 No disease produced. 



Do. 



Do. 



Do. 



As shown by Tables XI and XII Bacillus pluton is destroyed in 

 the j)resence of putrefactive processes. At incubator temperature 

 it resisted the effects of these processes for from 7 to 13 days and 

 at room temperature for from 21 to 35 days. 



During August and September, 1916, preliminary experiments 

 were made testing the resistance of Bacillus pluton to putrefaction 

 at outdoor temperature. The parasite was alive and virulent after 

 40 daj^s. The maximum period during which it will remain so has 

 not been determined. 



VIABILITY OF BACILLUS PLUTON IN HONEY 



Honey suspensions of Bacillus pluton from the stomach contents 

 of larvae sick or recently dead of European foulbrood were made and 

 distributed in flasks each containing about 300 c. c. These were 

 allowed to stand at room temperature shielded from the light. At 

 intervals thereafter colonies free from the disease were inoculated 



