32 . BULLETIN 817, TJ. S. DEPT. OF AGKICULTTJEE. 



Experiment No. 2. 



February 28, 1917 : Fed 5 mice with eggs of Ascaris suum on bread. 



j\Iarch 3, 1917 : Second feeding. 



March 10, 1917 : One mouse died with symptoms of pneumonia ; no post- 

 mortem. 



]\Iarch 14, 1917 : Two more mice died from pneumonia, 14 days after the 

 first feeding, 11 days after the second. The post-mortem showed a heavy in- 

 vasion of the lungs in both mice. In one of the mice no larvse Avere found in 

 the liver or small intestine, 5 larvse in the cecum ; in the other, 2 larvse in the 

 liver, 36 in the small intestine, 4 in the cecum. 



The larvfe in the lungs were of two rather distinct sizes evidently correspond- 

 ing to the different dates of infection. The larger size varied from 0.53 to 0.88 

 rhm., the smaller from 0.28 to 0.33 mm. in length. 



March 23, 1917 : Examined feces of remaining mice for larvae. Negative. 



March 26, 1917: Killed fourth mouse 26 days after first feeding, 23 days 

 after second feeding. Post-mortem : Lungs, 15 larvae 3 .3 to 1.4 mm, ; spleen, 

 2 larvje 0.8 mm. ; thyroid, 1 larva 0.7 mm. ; liver, 2 larvfe 0.8, 0.9 mm. ; small 

 intestine, 1 larva 1.2 mm. ; cecum, 7 larvse 1.6-1.9 mm. 



]\Iarch. 28, 1917 : Examined feces of fifth mouse. Negative. 



April 21, 1917 : Killed fifth mouse. Negative, 



Experiment No. 3. 



April 27, 1917 : Fed 6 mice at 11 a. m. Ascaris suum eggs on bread. 



April 28, 1917: Killed 2 mice 28 hours after first placing food before them. 

 One negative; in small intestine of the other 1 Ascaris egg with shell intact, 

 in stomach 4 Ascaris eggs containing living embryos. 



May 2, 1917: Killed third mouse 5 days after feeding. Several, larvae in 

 liver, 0.4 to 0.55 mm. long ; length of esophagus 0.1 to 0.3 mm., distance from 

 anus to tip of tail 0.07 mm., greatest width 0.02 mm. Spleen, lungs, heart, 

 kidney, stomach, small intestine, cecum, negative. 



May 5, 1917 : Killed fourth mouse 8 days after feeding. Larvae in liver only, 

 0.4 mm. long. 



May 10, 1917: Killed fifth mouse 13 days after feeding. Larvae in liver, 

 lungs, under peritoneum in various pai'ts of abdominal cavity, in feces. Larvae 

 in feces were 1.2 to 1.75 mm. long. Length of esophagus 0.18 to 0.23 mm. 

 Anus to tip of tail 0.1 mm. Larvae in lungs 1.7 mm. long. Length of esopha- 

 gus 0.24 mm. Anus to tip of tail 0.08 mm. Nerve ring 0.11 mm., excretory 

 pore 0.14 mm. from anterior end of body. Maximum width of body 0.06 mm. 



February 12, 1918 : Killed sixth mouse 9^ months after feeding. Negative 

 except for a few small black spots on the lungs, like the remains of old hem- 

 orrhages. 



Experiment No. 4. 



February 26, 1917 : Fed 8 mice Ascaris suum eggs on bread. 

 IMarch 2, 1917 : First mouse died 4 days after feeding. Negative. 

 March 17, 1917 : Killed second mouse 19 days after feeding. Seven larvae 

 in liver, 8 in spleen, none in other organs. 



March 26, 1917: Killed third mouse 28 days after feeding. Negative. 

 April 19, 1917 : Killed fourth mouse 52 days after feeding. Negative. 

 July 5, 1917 : Killed fifth mouse 129 days after feeding. Negative. 

 November 10, 1917: Killed sixth mouse 257 days after feeding. Negative. 

 December 3, 1917 : Killed seventh mouse 280 days after feeding. Negative. 



