34 BIILLETIISr 817, U. S. DEPT. OF AGKICULTURE. 



October 5, 1917 : Fourth mouse died 13 days after feeding. Not exauiined. 



October 6, 1917 : Two remaining mice died 14 days after feeding. Lai'vie 

 found in lungs. 



October 9, 1917 : The 2 mice bom October 4 were examined and found free 

 from infection. 



Experiment No. 7. 



November 4, 191 7 : A half-grown cat was fed many thousands of eggs of 

 Ascaris suum. 



November 12, 1917 : Cat chloroformed and examined 8 days after feeding. 

 Lungs, liver, spleen, small intestine, trachea, and pharynx examined and found 

 negative. . 



Experiment No. 8. 



August 21, 1916 : Two rats were fed bread soaked in a culture of Belascaris 

 marginaUi incubated for 33 days in weak formalin. 



August 22, 1916 : Feces of rats contained Belascaris eggs with unhatched 

 motile embryos, and a few hatched dead embryos. 



August 26, 1916: Killed one rat 5 days after feeding. Lung with hemor- 

 rhagic petechiee. One active larva found. Other organs not thoroughly ex- 

 amined. 



August 29, 1916 : Killed second rat 8 days after feeding. Lungs with hemor- 

 rhagic petechise but a hurried examination failed to reveal worms. 



Experiment No. 9. 



October 11, 1917: Fed 6 guinea pigs with culture of Ascaris suum, each 

 animal receiving two pipettes- — about 2.'4 cubic centimeters — of the culture. The 

 animals were also allowed to eat oats over which a culture of Ascaris eggs had 

 been poured. 



October 16, 1917 : Killed the first guinea pig 5 days after feeding. The post- 

 mortem showed numerous larvae in the lungs, 1 larva in trachea, none in liver, 

 spleen, esophagus, and small intestine. 



October 17, 1917 : Second guinea pig died 6 days after feeding. Lungs in- 

 tensely hemorrhagic (color of beef liver). Larvae very numerous in lungs; 

 numerous in trachea. Esophagus, stomach, liver, spleen, small intestine all 

 negative. The larvae in the lungs varied in length from 0.35 to 0.69 mm., with 

 esophagus 0.12 to 0.15 mm. in length. Those in the. trachea were considerably 

 larger, 0.71 to 1.13 mm. in length, 0.035 to 0.04 mm. in width, with esophagus 

 0.13 to 0.15 mm. long. 



October 17, 1917 : Third guinea pig died, 6 days after feeding. Lungs in- 

 tensely hemorrhagic. Larvae were very numerous in the lungs, numerous in 

 the trachea, several in the pharynx, 2 in the esophagus. Liver, spleen, stomach, 

 small intestine all negative. Large intestine not examined. The larvae in the 

 pharynx varied in length from 0.6 to 1.02 mm., the largest measuring 0.045 mm. 

 in width, with esophagus 0.19 mm. long. 



October 18, 1917 : Fourth guinea pig died from pneumonia 7 days after 

 feeding. Numerous larvae in lungs, several in trachea, 2 in the stomach 

 0.49 and 0.78 mm. in length, both dead and degenerated, possibly from the 

 action of the digestive juices. Small intestine, liver, thyroid. Fallopian tubes, 

 pharynx, all negative. Esophagus not examined. 



October 19, 1917 : Fifth guinea pig died from pnevimonia 8 days after feed- 

 ing. Larvae very numerous in lungs, numerous in trachea and pharynx, not 

 found in esophagus, liver, thyroid, submaxillary glands, stomach, and small 

 intestine. 



