6 



BULLETIN 819, U. S. DEPAETMENT OF AGKICULTURE. 



Later in the course of the work trips were made to the oyster beds, 

 where the samples were taken just as the oysters were brought out 

 of the water, thus avoiding the contact of the oyster with the deck, 

 which was known to be infected. Such samples, placed in card- 

 board boxes, were kept in a cool place until they could be taken to the 

 laboratory and analyzed. This was usually several hours later, but, 

 inasmuch as the analysis was qualitative rather than quantitative, 

 the delay has not affected the results. 



Table 2. — Analyses of oysters from the hay. 



Date. 



1916. 

 Nov. 12. 

 Dec. 4... 



1917. 

 Jan. 8... 

 Jan. 30.. 

 Feb. 5... 

 Feb. 21.. 

 Mar. 1... 

 Mar. 12.. 

 Mar. 15.. 

 Mar. 19.. 

 Mar. 22.. 

 Mar. 26.. 

 Mar. 29.. 

 Apr. 4... 

 Apr. 12. . 

 Apr. 26.. 

 May 24.. 

 May 29. . 

 May 31.. 

 June 4. . 

 June 8. . 

 J\mel2.. 

 June 14.. 

 June21.. 

 Jxme26.. 

 June 28.. 

 July 3. . . 

 Julys... 

 July 9... 

 July 11.. 

 July 16.. 

 July 19.. 

 July 24.. 

 July 31.. 

 Sept. 13. 

 Do.. 



Source. 



Steamer: 

 B.... 



A 



A 



A ^ 



E 



B 



B 



B 



C 



A 



Schooner from Long Island 



Steamer D 



Dredge on A, as taken from water 



do 



do 



Dredge on C, as taken from water . . . .' 



Seed oysters taken back from the shop during winter. Taken from bed 4 



Old oysters taken at bed 6 



do 



.do. 

 .do. 



Oysters taken at bed 2 



Old oysters taken at bed 6 



Oysters taken at bed 4 



do 



Deck of Steamer B 



Old oysters taken at bed 5 



Oysters taken at bed 2 



do 



Oysters taken at bed 6 



Oysters taken at bed 3 



Young oysters clinging to starfish mops at bed 2. 



do 



Oysters taken from bed 4 



Oysters taken at bed 6 



Result. 







1 colony. 















1 







1 







2 



colony. 

 I 

 colony. 



colonies. 

 Do. 



colonies. 

 Do. 



colony. 



colonies. 



colony. 



Results. 



The results recorded in Table 2 show that the pink yeasts may also 

 be found in oysters before they have been handled or brought in 

 contact with infected objects. The number of positive results was 

 not as great in the case of the oyster analyses as in the case of analy- 

 ses made from the oyster house, only 10 oyster samples of the 38 

 examined, or 26.3 per cent, showing the presence of the yeasts. 

 There was also a decided difference in the number of colonies to 

 be found on the plates made from samples from the two sources. 



